阿维A对寻常性银屑病患者树突状细胞的影响

发布时间：2018-04-13 01:36

  本文选题：银屑病 + 流式细胞术　； 参考：《中国皮肤性病学杂志》2014年01期

【摘要】：目的研究阿维A调控银屑病患者外周血树突状细胞功能,探讨阿维A治疗银屑病作用机制。方法抽取银屑病患者外周血,分离单个核细胞,体外诱导培养树交状细胞(Dendritic cells,DCs)。不同浓度阿维A(Inmol/L和10nmol/L)作用DCs 24h,对照组为只加培养基,流式细胞术(Flow cytometry,FCM)检测DCs中CD80,CD83及CD86表达水平,酶联免疫吸附试验(ELISA)方法检测细胞培养上清液中IL-12表达水平。结果体外成功培养出DCs,流式细胞术检测到1,10nmol/L阿维A作用DCs24h后,CD80,CD83及CD86表达率分别为(69.74±3.83)%、(61.12±3.03)%、(70.18±3.32)%和(60.19±3.97)%、(50.78±4.81)%、(48.96±3.18)%,显著低于对照组DCs上CD80,CD83及CD86表达率(75.15±2.76)%、(72.43±1.94)%、(77.81±2.46)%(P0.01)。1,10nmol/L阿维A作用DCs 24h后,DCs分泌到细胞培养液中的IL-12表达水平分别为(260.36±15.26)(226.98±17.24)显著低于对照组(282.80±23.26)(P0.01)。结论阿维A可以降低银屑病患者外周血中DCs上CD80.CD83及CD86表达率,抑制DCs活化,且降低IL-12因子的分泌量,能够调控患者机体的免疫功能,从而对银屑病起到治疗作用。
[Abstract]:Objective to study the regulation of Avera on the function of peripheral blood dendritic cells in patients with psoriasis and to explore the mechanism of Avera in the treatment of psoriasis.Methods Mononuclear cells were isolated from peripheral blood of patients with psoriasis, and dendritic cells were cultured in vitro.DCs was treated with Avi A(Inmol/L and 10nmol / L for 24 h. Flow cytometry was used to detect the expression of CD80 CD83 and CD86 in DCs. Elisa was used to detect the expression of IL-12 in the supernatant of cell culture. The expression of CD80 CD83 and CD86 in the supernatant of cell culture was detected by flow cytometry (FCM) and enzyme linked immunosorbent assay (Elisa).缁撴灉浣撳�栨垚鍔熷煿鍏诲嚭DCs,娴佸紡缁嗚優鏈�妫�娴嬪埌1,10nmol/L闃跨淮A浣滅敤DCs24h鍚，

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