SD大鼠外阴慢性单纯性苔藓模型的构建及蛋白酶激活受体2的表达

发布时间：2018-04-14 14:10

本文选题：外阴慢性单纯性苔藓 + 动物模型　；参考：《南方医科大学学报》2017年01期

【摘要】：目的建立SD大鼠外阴慢性单纯性苔藓(Lichen simplex chronicus,LSC)动物模型,研究蛋白酶激活受体2(protease activated receptor 2,PAR2)在模型大鼠外阴皮损中的表达,为LSC提供理想的动物模型。方法将70只雌性SD大鼠随机分为A组(空白对照组,n=10)、B组(丙酮对照组,n=10)和C组(单纯机械刺激对照组,n=10)、D组(实验组,n=40)。A组不予以任何处理;B组每周3次涂丙酮溶液于大鼠外阴皮肤,共10周;C组每周3次对大鼠皮肤予以单纯的机械刺激,共10周;D组予以0.5%二甲基苯并蒽(7,12-Dimethylbenzanthracene,DMBA)丙酮溶液联合机械刺激每周3次诱导大鼠外阴皮肤,共10周。6、8、10、12、14周肉眼及镜下观察大鼠外阴皮肤的变化。免疫组织化学、Western blotting检测A组和D组PAR2蛋白的表达,q RT-PCR检测PAR2的m RNA的表达。结果 D组第8周有57.5%(23/40)的SD大鼠出现了LSC改变,第10周有95%(38/40)的大鼠外阴表现为LSC,至12周时,20%(8/40)的大鼠外阴都形成了乳头状新生物。丙酮及单纯的机械刺激无导致LSC的作用。免疫组化及Western blotting结果显示D组PAR2的蛋白表达量较A组明显增加(P0.05);q RT-PCR结果显示D组PAR2的m RNA的表达量较A组明显增加(P0.05)。结论机械刺激联合0.5%DMBA丙酮溶液可诱导雌性SD大鼠LSC,PAR2可能与疾病的发生及进展密切相关。
[Abstract]:Objective to establish an animal model of chronic lichen vulva simplex (Lichen simplex chronicusus) in SD rats, and to study the expression of 2(protease activated receptor 2 (PAR2) in vulvar lesions of the model rats, so as to provide an ideal animal model for LSC.Methods 70 female Sprague-Dawley rats were randomly divided into two groups: group A (control group), group B (acetone control group, n = 10) and group C (control group with mechanical stimulation), group D (n = 40). Group A was treated with acetone solution 3 times a week without any treatment.In rat vulva skin,The rats in group C were treated with mechanical stimulation three times a week for 10 weeks, and group D were treated with 0.5% dimethyl benzanthene 712-Dimethylbenzanthracene-DMBA solution combined with mechanical stimulation three times a week to induce vulvar skin.The changes of vulvar skin in rats were observed with naked eye and under microscope for 10 weeks.The expression of PAR2 protein in group A and D was detected by Western blotting. The expression of m RNA in PAR2 was detected by Q RT-PCR.Results in group D, 57.5% of SD rats had LSC changes at the 8th week, 95 / 38 / 40 of the rats' vulva showed LSC at the 10th week, and at 12 weeks, 20 / 40% of the vulva formed papillary neoplasm.Acetone and mechanical stimulation alone had no effect on LSC.Immunohistochemical and Western blotting results showed that the expression of PAR2 protein in group D was significantly higher than that in group A. The results showed that the expression of m RNA in PAR2 in group D was significantly higher than that in group A.Conclusion Mechanical stimulation combined with 0.5%DMBA acetone solution can induce LSC-PAR2 in female SD rats, which may be closely related to the occurrence and progression of the disease.
【作者单位】：重庆医科大学生物医学工程学院//省部共建国家重点实验室培育基地—重庆市超声医学工程重点实验室//重庆市生物医学工程学重点实验室//重庆市微无创医学协同创新中心;
【基金】：十二五科技支撑计划(2011BAI14B01)
【分类号】：R758.6;R-332

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