强脉冲光治疗紫外线所致皮肤光老化作用的机制研究

发布时间：2018-04-15 12:58

本文选题：紫外线 + 强脉冲光　；参考：《青岛大学》2010年硕士论文

【摘要】： 探讨强脉冲光治疗紫外线所致皮肤光老化的可能作用机制,为临床应用提供理论依据。 30只大鼠随机分为三组：正常对照组,UV照射组,IPL治疗组。正常组不施加任何处理因素,仅同步喂养；紫外线照射组仅照射紫外线；IPL治疗组为紫外线照射+IPL照射。采用HE染色观察各组皮肤组织病理改变,检测各组皮肤组织中MDA含量,SOD、GSH-pX、CAT活性,western blot检测皮肤组织中MMP-1、TGF--β1以及bFGF表达变化。 (1)正常对照组大鼠表皮组织结构完整,细胞分层清晰,真皮内弹性纤维和胶原纤维形态及走向正常；紫外线照射后,大鼠的表皮组织变薄,结构不甚完整,分层不清,表皮与真皮交界呈直线状,纤维组织发生变性、破坏,可见真皮组织排列紊乱,分布不均或稀疏,有较多的炎性细胞浸润；IPL治疗组大鼠皮肤组织受损程度较轻,表皮组织结构比较完整,细胞分层比较清晰,皮肤厚薄不甚均一,真皮内弹性纤维和胶原纤维形态及走向基本正常。 (2)UV照射组大鼠皮肤组织中MDA含量较正常皮肤组织显着增加,而皮肤组织中SOD、GSH-pX、CAT含量较正常皮肤组织显着降低,P0.05。IPL治疗后,大鼠皮肤组织中MDA含量较UV照射组大鼠显着降低,而皮肤组织中SOD、GSH-pX、CAT含量较UV照射组大鼠显着增高,P0.05。 (3)UV照射组大鼠皮肤组织中MMP-1蛋白表达较正常皮肤组织显着增加,而皮肤组织中TGF-β1、bFGF含量较正常皮肤组织显着降低,P0.05。IPL治疗后,大鼠皮肤组织中MMP-1蛋白表达较UV照射组大鼠显着降低,而皮肤组织中TGF-β1、bFGF蛋白表达较UV照射组大鼠显着增高,P0.05。 (1)长期紫外线照射导致大鼠发生光老化。 (2)长期紫外线照射可致皮肤组织中产生脂质过氧化而致氧化应激,使MDA含量升高,SOD、GSH-px、CAT活性降低,可致皮肤组织中MMP-1蛋白含量增高。 (3)IPL可治疗长期紫外线辐射所致的光老化,改善皮肤的外观,降低皮肤组织中MDA含量,提高SOD、GSH-px、CAT活性。同时可使皮肤组织中MMP-1蛋白含量降低,增加TGF-β和bFGF蛋白含量。 (4)调节机体氧化损伤与抗氧化损伤动态的平衡,通过诱导有损伤修复作用的细胞因子TGF-β和bFGF表达,抑制参与皮肤组织胶原降解的MMP-1表达,可能是IPL治疗紫外线所致皮肤光老化的机制之一。
[Abstract]:To explore the possible mechanism of intense pulse light in treating photoaging of skin induced by ultraviolet ray, and to provide theoretical basis for clinical application.Thirty rats were randomly divided into three groups: normal control group treated with IPL.No treatment factors were applied in the normal group, and only simultaneous feeding was performed in the ultraviolet radiation group, and IPL irradiation was only used in the ultraviolet irradiation group.The pathological changes of skin tissue were observed by HE staining. The content of MDA and the activity of GSH-pXG cat in skin tissue were detected. The expression of MMP-1TGF- 尾 1 and bFGF in skin tissue were detected by western blot.1) in normal control group, the structure of epidermal tissue was intact, the layer of cells was clear, the shape and direction of elastic fiber and collagen fiber in dermis were normal, the epidermal tissue of rats was thinned, the structure was not complete, and the layer was not clear after ultraviolet irradiation.The junction of epidermis and dermis was linear, the fibrous tissue was degenerated and destroyed, the dermis tissue was arranged in disorder, distributed unevenly or sparsely, and there were more inflammatory cells infiltrating into the skin tissue of IPL treatment group, and the damage degree of skin tissue in IPL treatment group was less than that in IPL group.The structure of epidermis was relatively complete, the layer of cells was clear, the thickness of skin was not uniform, and the morphology and direction of elastic fibers and collagen fibers in dermis were normal.The content of MDA in skin tissue was significantly higher than that in normal skin tissue but the content of MDA in skin tissue was significantly lower than that in normal skin tissue after treatment with P0.05. IPL the content of MDA in skin tissue was significantly lower than that in UV irradiated group.The content of GSH-pXX cat in skin tissue was significantly higher than that in UV irradiation group (P 0.05).The expression of MMP-1 protein in the skin tissue was significantly increased than that in the normal skin tissue while the content of TGF- 尾 1 bFGF in the skin tissue was significantly lower than that in the normal skin tissue after treatment with P0.05. IPL the expression of MMP-1 protein in the skin tissue of the rats was significantly lower than that in the UV irradiated group.The expression of TGF- 尾 1 bFGF protein in skin tissue was significantly higher than that in UV irradiation group (P 0.05).Long-term ultraviolet radiation induced photoaging in rats.(2) Long-term ultraviolet irradiation could induce lipid peroxidation and oxidative stress in skin tissue, increase the content of MDA, decrease the activity of GSH-pxX cat, and increase the content of MMP-1 protein in skin tissue.It can treat the photoaging caused by ultraviolet radiation for a long time, improve the appearance of skin, decrease the content of MDA in skin tissue and increase the activity of GSH-pxX cat.At the same time, the content of MMP-1 protein in skin tissue was decreased, and the content of TGF- 尾 and bFGF protein was increased.(4) regulating the dynamic balance between oxidative and antioxidant injury, inhibiting the expression of MMP-1 involved in collagen degradation by inducing the expression of TGF- 尾 and bFGF.It may be one of the mechanisms of IPL in the treatment of ultraviolet-induced skin photoaging.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R758.1

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