普萘洛尔治疗婴幼儿血管瘤作用机制的动物实验研究

发布时间：2018-04-18 09:19

本文选题：血管瘤 + 普萘洛尔　；参考：《安徽医科大学学报》2014年09期

【摘要】：目的探讨普萘洛尔对人增生期血管瘤裸鼠种植瘤生长的影响。方法将手术切除的1例儿童增生期毛细血管瘤瘤体组织分切成组织块,分别植入16只幼小裸鼠(BALB/c nu/nu)的皮下,每只4处。建立人增生期血管瘤裸鼠模型,于第45天随机分成两组:普萘洛尔组的每只裸鼠用0.01%普萘洛尔溶液0.4 ml灌胃,每天1次,连续1周;给予同样体积的蒸馏水每天1次,连续1周灌胃作为对照组。密切观察裸鼠及移植瘤体的生长情况并做相应记录,通过免疫组织化学方法以及Western blot方法分别检测血管内皮生长因子(VEGF)及胰岛素样生长因子-2(IGF-2)的表达情况。结果普萘洛尔组裸鼠的肿瘤体积在干预期内明显下降,而对照组瘤体体积增加。使用普萘洛尔后,移植血管瘤VEGF、IGF-2蛋白的表达明显减弱。结论普萘洛尔可以有效地抑制人血管瘤移植瘤生长速度,抑制VEGF和IGF-2的分泌,从而有效抑制肿瘤血管生成,加速肿瘤转化为消退期。
[Abstract]:Objective to investigate the effect of propranolol on the growth of human proliferative hemangioma in nude mice.Methods the tumor tissues of 1 child with proliferative capillary hemangioma were divided into tissue blocks and implanted subcutaneously in 16 young nude mice with BALB / c nu-nu respectively.A nude mouse model of human hyperplastic hemangioma was established and randomly divided into two groups on the 45th day: each nude mouse in the propranolol group was given 0. 01% propranolol solution once a day for 1 week, and the same volume of distilled water was given once a day.The rats were treated as control group by gastric perfusion for 1 week.The expression of vascular endothelial growth factor (VEGF) and insulin-like growth factor-2IGF-2 (IGF-2) were detected by immunohistochemistry and Western blot.Results the tumor volume in the propranolol group decreased significantly during the intervention period, but increased in the control group.The expression of VEGF-IGF-2 protein in transplanted hemangioma was significantly decreased after propranolol.Conclusion Propranolol can effectively inhibit the growth rate of human hemangioma xenografts, inhibit the secretion of VEGF and IGF-2, thus effectively inhibit tumor angiogenesis and accelerate the process of tumor transformation into extinction.
【作者单位】：安徽医科大学第二附属医院整形外科;
【基金】：安徽省科技厅项目(编号:KJ2011Z190)
【分类号】：R739.5

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