发光二极管630nm红光和460nm蓝光照射对日本大耳白兔皮肤创面愈合的影响

发布时间：2018-04-18 18:09

本文选题：发光二极管 + 皮肤创伤　；参考：《中国医学科学院学报》2017年03期

【摘要】：目的观察发光二极管(LED)630 nm红光和460 nm蓝光照射对日本大耳白兔皮肤创面愈合的影响。方法采用日本大耳白兔8只,建立兔背部皮肤创伤模型,每只兔子3个伤口,分别给予红、蓝光距离15 cm垂直照射(15 min/次)和自然愈合处理。照射至创伤后第21天,观察各组创面愈合数目、愈合面积,计算创伤愈合面积百分比,比较两种光源的治疗作用;HE染色观察新生组织结构;Masson染色观察胶原纤维增生情况;免疫组织化学分析新生皮肤纤维细胞生长因子(FGF)、表皮生长因子(EGF)、内皮细胞生长因子(CD31)、增殖细胞核抗原(Ki-67)及炎性细胞因子(CD68)的表达情况。结果红光组、蓝光组和对照组的愈合率分别为50.0%(4/8)、25.0%(2/8)和12.5%(1/8)。自造模后第12天起,红光组伤口的愈合面积百分比均明显高于蓝光组及对照组(P0.05或P0.01)。建模后第21天,红光组新生皮肤厚度为(2.95±0.34)mm,明显高于对照组的(2.52±0.42)mm(F=3.182,P=0.016)。胶原纤维平均光密度为0.15±0.03,明显高于蓝光组的0.09±0.01(F=7.316,P=0.012)和对照组的0.07±0.01(F=7.316,P=0.003)。免疫组织化学检测结果显示,红光组的EGF、FGF、CD31抗原、Ki-67表达较蓝光组和对照组明显增多,CD68较蓝光组和对照组明显减少(P0.05或P0.01)。结论 LED红光照射可促进日本大耳白兔皮肤创面愈合,其可能是通过引起皮肤表皮细胞和血管内皮细胞生长及纤维组织增生来促成的。
[Abstract]:Objective to observe the effects of red light (630 nm) and blue light (460 nm) on the healing of skin wounds in Japanese white rabbits.Methods 8 Japanese white rabbits were used to establish the model of skin injury on the back of rabbits. Each rabbit was treated with red and blue light for 15 min/ and natural healing.On the 21st day after irradiation, the number and area of wound healing were observed, and the percentage of wound healing area was calculated. The therapeutic effects of the two light sources were compared with HE staining to observe the proliferation of collagen fibers by Masson staining.The expression of fibroblast growth factor (FGFF), epidermal growth factor (EGFN), endothelial cell growth factor (CD31), proliferating cell nuclear antigen (Ki-67) and inflammatory cytokine (CD68) were analyzed by immunohistochemistry.Results the healing rates of red light group, blue light group and control group were 50. 0% and 25. 0% and 12. 5% 8 / 8 respectively.Since the 12th day after modeling, the percentage of wound healing area in red light group was significantly higher than that in blue light group and control group (P0.05 or P0.01).On the 21st day after modeling, the thickness of newborn skin in the red light group was 2.95 卤0.34 mm, which was significantly higher than that in the control group (2.52 卤0.42 mm).The average light density of collagen fibers was 0.15 卤0.03, which was significantly higher than that of the blue light group (0.09 卤0.01) and the control group (0.07 卤0.01) and the control group (0.07 卤0.01).The results of immunohistochemistry showed that the expression of Ki-67 in EGF- FGFGF-CD31 antigen in red light group was significantly higher than that in blue light group and control group. The expression of CD68 in red light group was significantly lower than that in blue light group and control group (P0.05 or P0.01).Conclusion LED red light irradiation can promote the healing of skin wounds in Japanese white rabbits, which may be induced by the growth of skin epidermal cells and vascular endothelial cells and the proliferation of fibrous tissue.
【作者单位】：中国医学科学院北京协和医学院医学实验动物研究所卫生部人类疾病比较医学重点实验室&国家中医药管理局人类疾病动物模型三级实验室;中国人民解放军火箭军总医院皮肤科;
【基金】：十二五重大科技专项(2012ZX10004-501) 国家高技术研究发展计划(863计划)课题子任务(2003AA123310)~~
【分类号】：R751.05

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