卡泊三醇对咪喹莫特诱导的小鼠银屑病模型的影响

发布时间：2018-04-23 05:18

本文选题：卡泊三醇 + 银屑病　；参考：《临床皮肤科杂志》2017年12期

【摘要】：目的:探讨卡泊三醇对咪喹莫特诱导的小鼠银屑病模型的治疗作用及机制。方法:BALB/c小鼠随机分为3组:空白对照组、咪喹莫特组和卡泊三醇组。咪喹莫特组小鼠背部每日涂抹5%咪喹莫特乳膏,卡泊三醇组小鼠背部每日先涂抹5%咪喹莫特乳膏,6 h后再涂抹卡泊三醇搽剂,空白对照组每日涂抹凡士林作为对照。采用银屑病皮损面积与严重度指数(PASI)评分观察各组小鼠皮损变化情况;光镜观察皮损组织形态学变化;免疫病理分析各组皮损组织中淋巴细胞、中性粒细胞和树突状细胞浸润情况;实时荧光定量聚合酶链反应(q-PCR)对小鼠皮损组织中炎性细胞因子白细胞介素(IL)-17和干扰素(IFN)-γ的表达水平进行检测。结果:与咪喹莫特组比较,卡泊三醇组银屑病样皮损变化显著减轻,临床症状评分(clinical score,CS)降低,差异有统计学意义(P0.05);与咪喹莫特组比较,卡泊三醇组表皮厚度明显变薄,银屑病病理评分(Baker)明显降低,差异有统计学意义(P0.05);免疫病理结果显示卡泊三醇组皮损中的淋巴细胞、中性粒细胞和树突状细胞均低于咪喹莫特组,卡泊三醇组IL-17和IFN-γ表达水平与咪喹莫特组比较,差异均有统计学意义(P0.05)。结论:卡泊三醇具有一定的抗银屑病作用;该作用可能与降低皮损处的IL-17和IFN-γ表达水平有关。
[Abstract]:Aim: to investigate the therapeutic effect and mechanism of carpotriol on mice psoriasis induced by imiquimod. Methods Twenty BALB / c mice were randomly divided into three groups: blank control group, Imiquimod group and carpotriol group. The mice in the imiquimod group were smeared with 5% Imiquimod cream daily, the mice in the carpotriol group were first applied with 5% Imiquimod cream for 6 hours, then the carpotriol liniment was applied on the back of the mice, and vaseline was applied as the control in the blank control group. The area and severity index (PASI) of psoriasis lesions were used to observe the changes of skin lesions in each group, the histomorphologic changes of lesions were observed by light microscope, the lymphocytes in the lesions of each group were analyzed by immunopathology. The infiltration of neutrophils and dendritic cells and the expression of inflammatory cytokine IL-17 and IFNN- 纬 in mouse skin lesions were detected by real-time quantitative polymerase chain reaction (QPCR). Results: compared with the imiquimod group, the changes of psoriatic lesions and the clinical symptom score were significantly reduced in carpotriol group (P 0.05), and the epidermis thickness of carpotriol group was significantly thinner than that of imiquimod group. The pathological score of psoriasis decreased significantly (P 0.05), and the immunopathologic results showed that lymphocytes, neutrophils and dendritic cells in carpotriol group were lower than those in Imiquimod group. The expression of IL-17 and IFN- 纬 in carpotriol group was significantly higher than that in imiquimod group (P 0.05). Conclusion: carpotriol has a certain antipsoriatic effect, which may be related to the decrease of IL-17 and IFN- 纬 expression in lesions.
【作者单位】：成都市第二人民医院皮肤科;成都市青白江区人民医院;
【分类号】：R-332;R758.63

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