上海健康人群夏冬两季皮肤真菌群落组成:基于高通量测序技术的研究

发布时间：2018-04-25 23:05

本文选题：皮肤真菌 + 高通量测序　；参考：《复旦学报(自然科学版)》2014年06期

【摘要】：皮肤真菌与人体皮肤的健康状态有着紧密联系.本实验应用高通量测序技术对8名健康白领工作者7处皮肤部位分夏、冬两季采集得到的样品进行真菌群落组成和结构多样性研究.应用高通量测序得到113 759条有效序列,分属真菌2个门的99个属.担子菌门(Basidiomycota)的马拉色菌属(Malassezia)在各样品中丰度最高,达48.2%;在额头、背部和鼻孔等部位丰度高于其他采样部位;夏季马拉色菌在男性样品中丰度高于女性.其次是念珠菌属(Candida),在前臂、手背和指缝等部位丰度较高,背部则含量极少;女性样品中念珠菌丰度夏季远高于冬季,而男性中则随季节变化比较稳定.其他主要皮肤真菌有白粉菌属(Erysiphe)、枝孢霉菌属(Cladosporium)、链格孢霉属(Alternaria)和曲霉属(Aspergillus)等.多样性分析结果显示季节和皮肤部位是影响皮肤真菌群落组成的两个主要因素,性别的影响力相对较低.聚类分析结果表明,受试者自身皮肤真菌群落结构随季节变化的差异比同季节内各受试者之间的差异还要大.
[Abstract]:Skin fungi are closely related to the healthy state of human skin. In this study, high-throughput sequencing technique was used to study the community composition and structural diversity of fungi collected from 7 skin parts of 8 healthy white-collar workers in summer and winter. 113,759 effective sequences were obtained by high throughput sequencing, belonging to 99 genera of 2 phylum fungi. Malassezia (Basidiomycota) of basidiomycota had the highest abundance of 48.2 in all samples, higher abundance in forehead, back and nostrils, and higher abundance of Malassezia in male samples in summer than in women. The Candida genus Candida had higher abundance in forearm, back of hand and finger seam, but little content in back. The abundance of Candida in female samples in summer was much higher than that in winter, while that in men was more stable with the seasons. Other major cutaneous fungi include Erysipheus, Cladosporium, Alternaria and Aspergillus. The results of diversity analysis showed that season and skin position were the two main factors affecting the composition of skin fungi community, and the influence of sex was relatively low. The results of cluster analysis showed that the variation of fungal community structure with seasonal variation was greater than that between the subjects in the same season.
【作者单位】：复旦大学生命科学学院微生物学与微生物工程系;强生(中国)有限公司研发部;
【分类号】：R756

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