寻常型银屑病患者外周血中细胞因子的表达及其相关性研究

发布时间：2018-04-27 03:44

本文选题：寻常型银屑病 + 细胞因子　；参考：《广州中医药大学》2011年硕士论文

【摘要】：背景银屑病是皮肤科的常见病、多发病,男女均可发病,其确切的发病机制尚未完全阐明,涉及遗传、免疫、炎症、神经介质、细胞增殖与凋亡等多种因素。目前多数学者认为,银屑病是一种免疫诱导、过度再生性增殖性皮肤病。超抗原的刺激、T淋巴细胞、T细胞与KC相互作用、细胞因子异常、STAT3途径、免疫细胞膜分子异常、Toll受体以及神经免疫等在银屑病发病中起着重要作用。目的初步探讨银屑病患者血清中MCP-1、MIP-1α、SCD40L、TNF-α、EGF、IL-8、IL-10、IL-17及VEGF水平与中医证型、疾病严重程度、疾病分期间的关系。方法采用酶联免疫吸附方法(ELISA)对62例银屑病患者和20例健康人血清中细胞因子(MCP-1、MIP-1α、SCD40L、TNF-α、EGF、IL-8、IL-10、VEGF)进行测定,并对其与各中医证型的关系进行分析。结果 1.EGF呈高表达,与健康对照组比较,无统计学意义(P0.05)；中医各证型间比较,无统计学意义(P0.05)；与疾病分期、PASI评分无关(P0.05)；与病程有关(P0.01)。 2.IL-8呈高表达,血瘀证组最具代表意义,血瘀证组与健康对照组、血燥证组与健康对照组比较,有显著性差异(P0.01)；与病情活动、PASI评分、病程无关(P0.05)。 3.IP-10呈高表达,血瘀证组最具代表意义,血瘀证组与健康对照组、血燥证组与健康对照组比较,有显著性差异(P0.01)；与年龄、性别有关(P0.05)；与疾病分期、PASI评分、病程无关(P0.05)。 4. MCP-1呈高表达,与健康对照组比较,无统计学差异(P0.05)；血瘀证组与健康对照组比较,有统计学意义(P0.05)；与性别有关(P0.05)；与疾病分期、PAS I评分、病程无关(P0.05)。 5. MIP-1α呈高表达,与健康对照组比较有显著性差异(P0.01)；血瘀证组与健康对照组、血燥证组与健康对照组比较有显著性差异(P0.01)；与疾病分期、PASI评分、病程无关(P0.05)。 6. TNF-α呈高表达,与健康对照组比较有显著性差异(P0.01)；血瘀证组与健康对照组、血燥证组与健康对照组比较有显著性差异(P0.01)；与疾病分期、PASI评分、病程无关(P0.05)。 7. VEGF呈高表达,与健康对照组比较,无统计学意义(P0.05)；中医辩各证型间比较,无统计学意义(P0.05)；与病程有关(P0.05)；与疾病分期、PASI评分无关(P0.05)。 8.IL-17呈低表达,与健康对照组比较,无统计学意义(P0.05)；中医各证型间比较,无统计学意义(P0.05)；消退期与稳定期、消退期与进行期间比较,有统计学意义(P0.01)；与PAS I评分、病程无关(P0.05)。 9. SCD40L呈低表达,血燥证组最具代表性,与健康对照组比较,有统计学意义(P0.05)；与疾病分期、PAS I评分、病程无关(P0.05)。结论 1.EGF在银屑病患者血清中高表达,且与病程有关。 2.IL-8在银屑病患者血清中高表达,其检测可能用于血瘀证、血燥证型银屑病与其他证型银屑病的中医辩证分型。 3.IP-10在银屑病患者血清中高表达,其检测可能用于血瘀证、血燥证型银屑病与其他证型银屑病的中医辩证分型,其表达水平与年龄、性别有关。 4.MCP-1在银屑病患者血清中高表达,其检测可能用于血瘀证型银屑病与其他证型银屑病的中医辩证分型,其表达水平与性别有关。 5. MIP-1α在银屑病患者血清中高表达,其检测可能用于血瘀证、血燥证型银屑病与其他证型银屑病的中医辩证分型。 6. TNF-α在银屑病患者血清中高表达,其检测可能用于血瘀证、血燥证型银屑病与其他证型银屑病的中医辩证分型。 7. VEGF在银屑病患者血清中高表达,与病程有关。 8.IL-17在银屑病患者血清中低表达,与疾病活动有关。 9. SCD40L在银屑病患者血清中低表达,其检测可能用于血燥证型银屑病与其他证型银屑病的中医辩证分型。 10.细胞因子表达异常在银屑病的发病过程中起重要作用,异常的细胞因子间存在某些正向调控作用。
[Abstract]:background
Psoriasis is a common disease in the Department of dermatology. The pathogenesis of male and female is more common. The exact pathogenesis of psoriasis is not fully elucidated. It involves many factors, such as heredity, immunity, inflammation, nerve medium, cell proliferation and apoptosis. Most scholars believe that psoriasis is an immune induced, excessively regenerative proliferative dermatosis. Hyper antigen stimulation, T lymph nodes The interaction of cells, T cells with KC, abnormal cytokine, STAT3 pathway, immune cell membrane molecular abnormalities, Toll receptor and neuroimmunology play an important role in the pathogenesis of psoriasis.
objective
The relationship between the levels of MCP-1, MIP-1 a, SCD40L, TNF- a, EGF, IL-8, IL-10, IL-17 and VEGF in the serum of psoriasis patients with TCM syndrome, the severity of disease and the staging of disease.
Method
The serum levels of cytokines (MCP-1, MIP-1 a, SCD40L, TNF- a, EGF, IL-8, IL-10, VEGF) in 62 patients with psoriasis and 20 healthy people were measured by ELISA, and the relationship between them and TCM syndrome types was analyzed.
Result
The high expression of 1.EGF was not statistically significant (P0.05) compared with the healthy control group (P0.05), and there was no statistical significance (P0.05) in different TCM syndromes (P0.05); it was not related to the staging of the disease (P0.05), and related to the course of the disease (P0.01).
2.IL-8 showed high expression, blood stasis syndrome group was the most representative, blood stasis syndrome group and healthy control group, blood dryness syndrome group compared with healthy control group, there was significant difference (P0.01), and the disease activity, PASI score, the course of the disease was not related (P0.05).
3.IP-10 showed high expression, blood stasis syndrome group was the most representative, blood stasis syndrome group and healthy control group, blood dryness syndrome group compared with healthy control group, there was significant difference (P0.01), related to age, sex (P0.05), and disease staging, PASI score, and the course of disease (P0.05).
4. MCP-1 was highly expressed, and there was no statistical difference compared with the healthy control group (P0.05). The blood stasis syndrome group was statistically significant (P0.05) compared with the healthy control group (P0.05); it was related to sex (P0.05); the disease staging, PAS I score, and the course of disease were not related (P0.05).
5. MIP-1 a high expression, compared with the healthy control group has significant difference (P0.01), blood stasis syndrome group and healthy control group, blood dryness syndrome group and healthy control group have significant difference (P0.01), and the disease stage, PASI score, the course of the disease is not related (P0.05).
6. TNF- a high expression, compared with the healthy control group has significant difference (P0.01), blood stasis syndrome group and healthy control group, blood dryness syndrome group and healthy control group have significant difference (P0.01), and the disease stage, PASI score, the course of the disease is not related (P0.05).
The high expression of 7. VEGF was not statistically significant (P0.05) compared with the healthy control group (P0.05), and there was no statistical significance (P0.05) between the TCM syndromes and the course of disease (P0.05), and the PASI score was not related to the staging of the disease (P0.05).
8.IL-17 showed low expression, no statistical significance (P0.05) compared with the healthy control group (P0.05); there was no statistical significance (P0.05) for each type of TCM syndrome. The period of regression and stability, the period of regression and the period were statistically significant (P0.01), and the course of disease was not related with PAS I score (P0.05).
9. SCD40L showed low expression, the blood dryness syndrome was the most representative, compared with the healthy control group, it was statistically significant (P0.05), and the disease staging, PAS I score, and the course of disease were not related (P0.05).
conclusion
1.EGF is highly expressed in sera of psoriasis patients, and is related to the course of disease.
2.IL-8 is highly expressed in sera of psoriasis patients, and its detection may be used for blood stasis syndrome, psoriasis of blood dryness type syndrome and TCM syndrome differentiation of psoriasis.
3.IP-10 is highly expressed in the serum of patients with psoriasis, and its detection may be used in the dialectical typing of blood stasis syndrome, psoriasis of blood dryness syndrome and other syndromes of psoriasis. The expression level is related to age and sex.
The high expression of 4.MCP-1 in the serum of patients with psoriasis may be used in the dialectical typing of psoriasis of blood stasis syndrome and other syndromes of psoriasis. The expression level of psoriasis is related to sex.
5. MIP-1 alpha is highly expressed in sera of psoriasis patients, and its detection may be used for blood stasis syndrome, psoriasis of blood dryness syndrome and TCM syndrome differentiation of other syndromes.
6. TNF- alpha is highly expressed in sera of psoriasis patients, and its detection may be used for blood stasis syndrome, psoriasis of blood dryness syndrome and TCM syndrome differentiation of other syndromes.
7. high expression of VEGF in serum of psoriasis patients is related to the course of disease.
8.IL-17 is low in psoriatic serum and is associated with disease activity.
9. the expression of SCD40L in serum of psoriasis patients is low, and its detection may be used in TCM syndrome differentiation of psoriasis due to blood dryness syndrome and other syndromes.
10. abnormal expression of cytokines plays an important role in the pathogenesis of psoriasis. There are some positive regulatory effects between abnormal cytokines.

【学位授予单位】：广州中医药大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R758.63

【参考文献】