KG软膏治疗急性湿疹的药效作用及部分机理研究

发布时间：2018-04-27 08:16

本文选题：KG软膏 + 急性湿疹　；参考：《成都中医药大学》2010年硕士论文

【摘要】：目的：观察KG软膏对二甲苯致小鼠耳廓急性炎症和2,4-二硝基氟苯(DNFB)所致迟发型超敏反应模型小鼠的影响,探讨治疗急性湿疹的药效及可能的作用机制。方法：1.昆明种健康雄性小鼠60只,18-22g,按体重分层随机分为6组,每组10只。设模型对照组、基质对照组、阳性对照组(地塞米松)、KG软膏高、中、低剂量六个组,连续4天给药,前三天每天给药一次,每次只涂左耳,第四天左右耳均给药,给药两次,末次给药后1h后,滴20ul二甲苯于鼠左耳,1小时后将小鼠断颈处死,沿耳廓基线部位剪下左右耳称重,计算两耳重量差异,即肿胀度。 2.昆明种健康雄性小鼠60只,18-22g,随机分为6组,分别为正常对照组、模型对照组组、KG软膏高剂量组、KG软膏中剂量组、KG软膏低剂量组,阳性对照组(地塞米松)。采用DNFB,腹部致敏、耳廓激发的方法建立小鼠迟发型超敏反应模型。处死小鼠后,沿耳廓基线部位剪下双耳,称重,并计算肿胀度；左耳用10%福尔马林溶液固定,HE染色,观察耳廓充血、水肿及炎症细胞浸润的程度；并采用免疫组化的方法观察KG软膏对小鼠的耳组织中IFN-γ、IL-4、TNF-α、ICAM-1表达的影响。结果：1.与模型组相比,高、中剂量组小鼠耳廓肿胀度均小于模型组,有统计学意义(P0.05或P0.01)；低剂量组无明显差异(P0.05),但有降低趋势。表明KG软膏对二甲苯所致小鼠急性耳肿胀有抑制作用,有抗急性炎症作用。 2.与模型组相比,各给药组小鼠耳廓肿胀度均小于模型组,有统计学差异(P0.01)；KG软膏各剂量组病理学评分均明显降低,有统计学意义(P0.01)；说明KG软膏可降低DNFB激发后小鼠左耳的肿胀度,减轻左耳病理损伤,对DNFB所致小鼠迟发超敏反应有抑制作用。 3.模型组与正常对照组比较,DNFB致敏小鼠耳组织中IFN-γ、IL-4、TNF-α、ICAM-1表达均明显增高,有统计学意义(P0.05或P0.01)；给药各组IFN-γ、IL-4、TNF-α、ICAM-1的表达水平降低,有统计学意义(P0.05或P0.01),说明KG软膏能抑制这些细胞因子的表达。结论：1.KG软膏能抑制二甲苯诱发的小鼠耳廓炎症反应,2,4-二硝基氟苯诱发的小鼠迟发型超敏反应、改善耳组织炎细胞浸润情况、减轻细胞间水肿,表明KG软膏有治疗急性湿疹的作用。 2.KG软膏治疗急性湿疹作用机理部分与其抑制损伤局部IFN-γ、IL-4、TNF-α、ICAM-1等细胞因子的表达、调节T细胞亚群功能有关。
[Abstract]:Objective: to observe the effect of KG ointment on acute inflammation of auricle induced by xylene and delayed type hypersensitivity induced by DNFB in mice, and to explore the effect of KG ointment on acute eczema and its possible mechanism. Method 1: 1. Sixty healthy Kunming male mice were randomly divided into 6 groups according to their body weight, 10 rats in each group. Model control group, matrix control group, positive control group (high, middle and low dose of dexamethasone KG ointment) were given for 4 days. One hour after the last administration, the mice were killed by 20ul xylene dropping in the left ear for 1 hour. The left and right ears were cut off to weigh the right and left ears along the base line of the auricle, and the difference of the weight between the two ears was calculated, that is, the swelling degree. 2. Sixty Kunming healthy male mice were randomly divided into 6 groups: normal control group, model control group, high dose KG ointment group, low dose KG ointment group, and positive control group (dexamethasone). The delayed type hypersensitivity model of mice was established by DNFB, abdominal sensitization and auricle stimulation. After the mice were killed, the ears were cut off along the base line of the auricle to weigh and calculate the swelling degree, and the left ear was fixed with 10% formalin solution to fix the HE staining to observe the degree of congestion, edema and inflammatory cell infiltration of the auricle. The effect of KG ointment on the expression of ICAM-1 in mouse ear tissue was observed by immunohistochemical method. The result is 1: 1. Compared with the model group, the swelling degree of auricle in the middle dose group was lower than that in the model group (P0.05 or P0.01), while in the low dose group there was no significant difference (P 0.05), but there was a tendency to decrease. The results showed that KG ointment could inhibit acute ear swelling induced by xylene and inhibit acute inflammation in mice. 2. Compared with the model group, the swelling degree of auricle in each administration group was lower than that in the model group, and the pathological score of each dose of KG ointment was significantly lower than that of the model group, indicating that KG ointment could reduce the swelling degree of left ear after DNFB stimulation. The delayed hypersensitivity reaction induced by DNFB could be inhibited by alleviating the pathological injury of left ear. 3. The expression of ICAM-1 in the ears of DNFB-sensitized mice was significantly higher than that in the normal control group (P 0.05 or P 0.01), and the expression level of IFN- 纬 IL-4TNF- 伪 -ICAM-1 was lower in each group than that in the control group, and the expression of ICAM-1 was significantly higher in the model group than that in the normal control group, and the expression of ICAM-1 in the ear tissue of the DNFB sensitized mice was significantly lower than that in the control group. There was significant difference (P 0.05 or P 0.01), indicating that KG ointment could inhibit the expression of these cytokines. Conclusion 1. KG ointment can inhibit the auricular inflammation induced by xylene in mice, improve the infiltration of ear tissue cells, and alleviate the edema of the cells, and inhibit the delayed hypersensitivity of mice induced by dimethylbenzene and dinitrofluorobenzene. It indicated that KG ointment had the effect of treating acute eczema. The mechanism of 2.KG ointment in the treatment of acute eczema is partly related to its inhibition of the expression of cytokines such as IFN- 纬 -IL-4, TNF- 伪 and ICAM-1, and the regulation of the function of T cell subsets.
【学位授予单位】：成都中医药大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R758.23

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