梅毒患者红细胞CR1密度相关基因多态性研究

发布时间：2018-04-29 10:08

  本文选题：梅毒 + 红细胞　； 参考：《兰州大学》2010年硕士论文

【摘要】： 梅毒是由梅毒螺旋体引起的一种危害性极大的慢性传染病,主要通过性接触和血液传播。梅毒的发病机制未完全阐明,出现血清固定的确切原因目前尚不清楚。当前对梅毒发病机制和血清固定原因的研究主要集中在白细胞免疫,认为梅毒发病与T细胞介导的免疫反应密切相关。近年来红细胞免疫逐渐成为感染性疾病的研究热点之一,有研究表明梅毒感染者的红细胞免疫功能较正常人明显降低,但关于梅毒患者CR1密度相关基因多态性的研究尚未见报道。 目的探讨梅毒患者红细胞CR1密度相关基因型的频率分布。 方法采用聚合酶链反应(PCR)、限制性内切酶HindⅢ酶切技术测定患者红细胞CR1密度相关基因多态性。 结果病例组中CR1基因HH、HL和LL基因型分布频率分别为58.75%、35%、和6.25%,对照组中HH、HL和LL基因型分布频率分别为68.75%、26.25%和5%,两组CR1基因型的分布频率差异无显著性(p0.05)；梅毒患者组基因突变率为41.25%,对照组为31.25%,也无显著性差异(p0.05)。 结论梅毒患者的红细胞免疫功能低下与红细胞CR1基因发生突变关系不密切。
[Abstract]:Syphilis is a serious chronic infectious disease caused by Treponema pallidum, mainly through sexual contact and blood transmission. The pathogenesis of syphilis is not fully clarified, and the exact cause of serum fixation is unclear. At present, the pathogenesis of syphilis and the cause of serum fixation are mainly focused on leukocyte immunity. It is believed that the pathogenesis of syphilis is closely related to T cell mediated immune response. In recent years, red blood cell immunity has gradually become one of the hotspots in infectious diseases. Some studies have shown that the red cell immune function of syphilis infected people is significantly lower than that of normal people. However, the study of CR1 density related gene polymorphism in syphilis patients has not been reported. Objective to investigate the frequency distribution of erythrocyte CR1 density related genotypes in syphilis patients. Methods Polymerase chain reaction (PCR) and restriction endonuclease (Hind 鈪，

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