CCL20在银屑病中的表达以及与缺氧的关系

发布时间：2018-04-30 07:57

本文选题：银屑病 + 缺氧　；参考：《华中科技大学》2011年硕士论文

【摘要】：银屑病是由环境因素激发的、遗传因素决定的慢性炎症皮肤病。而局部微环境的缺氧和表皮角质形成细胞的增殖是此疾病最重要的特征。我们的前期实验已经证明CCL20和HIF-1α在银屑病患者病损区活检组织中的表达较正常人明显增强。但是目前对CCL20在缺氧微环境中上调的机制还不甚明白。通过ELISA技术检测17例银屑病患者与6例正常人血清中CCL20水平后发现: CCL20在银屑病患者血清中的表达水平(26.76±19.34pg/ml)显著高于正常对照组(8.69±3.19 pg/ml),增高约3倍,有统计学意义,P0.05。本试验选用Hacat细胞作为正常角质形成细胞(KC)的模型,利用CO2培养箱(94%N2, 5%CO2, 1%O2)模拟银屑病患者皮损中局部缺氧微环境。通过RT-PCR技术检测发现表皮角质形成细胞缺氧6-12小时后,CCL20和HIF-1α表达明显增高,随后渐回到基线水平。此外我们还发现CCL20和HIF-1α的表达呈正相关(r = 0.855,p0.05)。因为将缺氧后的表皮角质形成细胞转染HIF-1αsiRNA下调HIF-1α表达后,CCL20在mRNA及蛋白水平的表达均回到基础水平。综上所述,CCL20可能在银屑病发病的免疫机制中发挥着重要的作用,CCL20的上调可能与HIF-1α有关,并且两者在银屑病发病中可能起协同作用。
[Abstract]:Psoriasis is a chronic inflammatory dermatosis caused by environmental factors and genetic factors. Hypoxia in local microenvironment and proliferation of epidermal keratinocytes are the most important characteristics of the disease. Our previous experiments have demonstrated that the expression of CCL20 and HIF-1 伪 in biopsy tissues of psoriatic lesions was significantly higher than that in normal controls. However, the mechanism of CCL20 upregulation in hypoxia microenvironment is not well understood. The expression of CCL20 in serum of 17 patients with psoriasis and 6 normal controls was detected by ELISA technique. It was found that the expression level of CCL20 in serum of psoriasis patients was significantly higher than that of normal controls (P = 26.76 卤19.34pg / ml) and 8.69 卤3.19pg / ml respectively, which was about 3 times higher than that of normal controls (P 0.05). In this experiment, Hacat cells were used as the model of normal keratinocytes (KC), and N2,5CO2,1O2 were used in CO2 incubator to simulate the local anoxic microenvironment in the skin lesions of psoriasis patients. The expression of CCL20 and HIF-1 伪 in epidermal keratinocytes was significantly increased after hypoxia for 6-12 hours, and then returned to baseline level. In addition, we also found a positive correlation between the expression of CCL20 and HIF-1 伪 (r = 0.855P 0.05). The expression of CCL20 in mRNA and protein levels of HIF-1 伪 siRNA was reduced to the basic level after transfection of hypoxia-induced epidermal keratinocytes (EKCs) to down-regulate the expression of HIF-1 伪. In conclusion, CCL20 may play an important role in the immune mechanism of psoriasis. The up-regulation of CCL20 may be related to HIF-1 伪, and both may play a synergistic role in the pathogenesis of psoriasis.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R758.63

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