一种新型辅酶Q10脂质载体系统在抗皮肤老化方面的研究

发布时间：2018-05-03 08:49

本文选题：辅酶Q10 + 纳米结构脂质载体　；参考：《清华大学》2010年硕士论文

【摘要】：皮肤光老化是皮肤衰老的重要原因之一,日光紫外线主要是通过诱导皮肤细胞产生活性氧自由基和氧化压力来损伤细胞和皮肤。辅酶Q10(CoQ10)是一种极具潜力的抗氧剂,然而由于其自身的难溶性特性限制了它在抗紫外线引起的皮肤光老化方面的应用。纳米结构脂质载体系统(NLC)是一种新型的给药系统,具有增加药物溶解能力、提高制剂稳定性、增强皮肤渗透能力和降低刺激性等优点。本课题的主要目的是设计并制备一种新型的经皮纳米给药系统来运载CoQ10,并对其各类功能性质进行研究和评价,期望能够得到一种高效、靶向、安全的经皮给药系统。为改善CoQ10在保护皮肤免受紫外线损伤方面的抗氧化效果,本课题制备了辅酶Q10纳米脂质载体系统(CoQ10-NLC)。同时,对制备的载药系统进行初步的物理化学性质的研究。利用激光散射仪、冷冻蚀刻电镜和低温冷冻电镜等技术研究了CoQ10-NLC的粒径、形貌和结构等特征。为更真实、准确的模拟自然状态的光老化,本课题采用人胚胎皮肤成纤维细胞(ESF-1)来建立体外细胞紫外损伤模型,ESF-1是一种原代培养的人皮肤细胞。对比添加普通CoQ10乳液组细胞,添加CoQ10-NLC的细胞在细胞活力、细胞形态和核形态方面都有明显的提高和改善。此外,添加CoQ10-NLC的细胞丙二醛(MDA,细胞膜脂质过氧化产物)水平只有添加CoQ10乳液组的61.5%。对比空白对照组,有CoQ10-NLC保护的细胞的超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-px)活性分别提高了81%和75%。在通过体外的细胞试验研究CoQ10-NLC载药系统在抗氧化方面的能力后,本课题又利用大鼠的背部皮肤作为模型来探讨其体内皮肤渗透性能。通过观察荧光染料尼罗红的特征荧光在皮肤纵切面上的分布来评估CoQ10-NLC克服皮肤屏障能力并在皮肤中的渗透效果,结果证明CoQ10-NLC较普通乳液具有更强的皮肤渗透能力。
[Abstract]:Skin photoaging is one of the important causes of skin aging. Sunlight ultraviolet rays mainly damage skin and cells by inducing the production of reactive oxygen free radicals and oxidative pressure. Coenzyme Q10 (CoQ10) is a potential antioxidant, but its insoluble properties limit its application in anti-UV induced photoaging of skin. NLCS is a new drug delivery system, which has the advantages of increasing drug dissolution ability, improving drug stability, enhancing skin permeability and reducing irritability. The main purpose of this paper is to design and prepare a novel transdermal nano-drug delivery system to carry CoQ10, and to study and evaluate its various functional properties in order to obtain an efficient, targeted and safe transdermal drug delivery system. In order to improve the antioxidation effect of CoQ10 in protecting skin from ultraviolet damage, CoQ10 nanometer lipid carrier system, CoQ10-NLCG, was prepared in this paper. At the same time, the physical and chemical properties of the drug loading system were studied. The particle size, morphology and structure of CoQ10-NLC were studied by means of laser scattering, freeze etching electron microscope and cryopreservation electron microscope. In order to simulate the photoaging of human embryonic skin fibroblasts (ESF-1), an in vitro ultraviolet damage model was established. ESF-1 is a primary cultured human skin cell. Compared with the normal CoQ10 emulsion group, the cell viability, cell morphology and nuclear morphology were significantly improved by adding CoQ10-NLC. In addition, the level of malondialdehyde (MDA), membrane lipid peroxidation product (MDAs) added with CoQ10-NLC was only 61.5 in CoQ10 emulsion group. Compared with the control group, the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) were increased by 81% and 75%, respectively. After studying the antioxidation ability of CoQ10-NLC drug carrier system in vitro, the rat back skin was used as a model to study the skin permeability in vivo. The distribution of characteristic fluorescence of fluorescent dye Nile red on the longitudinal surface of skin was observed to evaluate the ability of CoQ10-NLC to overcome the skin barrier and its permeation effect in the skin. The results showed that CoQ10-NLC had stronger skin permeability than ordinary emulsion.
【学位授予单位】：清华大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R758.1

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