辣椒辣素和苯酚对小鼠变应性接触性皮炎影响的研究

发布时间：2018-05-05 03:27

本文选题：神经因素 + 变应性接触性皮炎　；参考：《苏州大学》2011年硕士论文

【摘要】：目的: 观察辣椒辣素和苯酚对小鼠变应性接触性皮炎的影响,探讨神经系统与变应性接触性皮炎的关系和可能机理。方法: 选用雄性清洁级BALB/c小鼠48只,6-8周龄,将小鼠随机分为12组,以2,4-二硝基氯苯作为致敏剂于小鼠腹部致敏、右耳激发建立小鼠ACD模型。选用小鼠双侧耳厚差、耳重量差、组织切片显微镜下耳双面距离差、组织切片中浸润细胞的种类及数量、双侧耳引流淋巴结中淋巴细胞的增值活性等指标对小鼠ACD进行评价。实验①分别用特异性肽能神经毒性物质辣椒辣素和一般神经毒性化合物苯酚对小鼠行致敏前或激发前预处理,观察辣椒辣素和苯酚对ACD小鼠的传入支(致敏阶段)或传出支(激发阶段)的影响; 实验②在致敏前2天使用辣椒辣素,并于致敏后3天、6天、9天、12天、15天再激发,观察辣椒辣素对小鼠ACD的影响与时间的关系。结果: 1.小鼠ACD模型造模成功。模型组及各对照组小鼠双侧耳厚差、耳重量差、组织切片显微镜下耳双面距离差、单一核细胞数及多形核数目差及双侧耳后淋巴结中淋巴细胞的增值活性相比均有统计学差异(p0.01); 2.致敏前使用辣椒辣素或苯酚使局部皮肤神经纤维失活,或者系统性地使肽能神经失活后,双侧耳的各指标无统计学差异(p0.05),激发前用辣椒辣素使局部皮肤神经纤维失活,双侧耳的各指标无统计学差异(p0.05); 3.致敏前2天使用辣椒辣素,并于致敏后3天、6天、9天、12天、15天再激发,双侧耳的各指标的差异无统计学意义(p0.05)。结论: 1.辣椒辣素能阻止变应性接触性皮炎的形成,其机理是通过阻断小鼠变应性接触性皮炎的传入支(致敏阶段)和传出支(激发阶段)。 2.苯酚能阻断小鼠变应性接触性皮炎的传入支(致敏阶段)。 3.神经系统在小鼠变应性接触性皮炎的发生和发展中起着重要的作用。
[Abstract]:Objective: To observe the effects of capsaicin and phenol on allergic contact dermatitis in mice and to explore the relationship between nervous system and allergic contact dermatitis and its possible mechanism. Methods: Forty-eight male clean grade BALB/c mice aged 6-8 weeks were randomly divided into 12 groups. The mice were sensitized by 2-di-4-dinitrochlorobenzene in abdomen, and ACD model was established by right ear stimulation. The ACD of mice was evaluated by using the indexes of poor ear thickness, poor ear weight, the distance between the two sides of the ear under the microscope, the type and number of infiltrating cells in the tissue sections, and the proliferative activity of lymphocytes in the lymph nodes of the bilateral lateral ear drainage. In experiment 1, mice were pretreated with capsaicin, a specific peptidergic neurotoxic substance, and phenol, a general neurotoxic compound, before or before stimulation. The effects of capsaicin and phenol on the afferent branch (sensitizing stage) and efferent branch (excitation stage) of ACD mice were observed. In experiment 2, capsaicin was used 2 days before sensitization, and then stimulated 3 days, 6 days, 9 days, 12 days and 15 days after sensitization. The effect of capsaicin on ACD in mice was observed. Results: 1. Mouse ACD model was successfully established. In the model group and the control group, there were significant differences in the thickness of the ears, the weight of the ears, the distance between the two sides, the number of mononuclear cells and the number of polymorphic nuclei, and the proliferative activity of lymphocytes in the lymph nodes of the posterior lymph nodes of the two lateral ears. 2. After using capsaicin or phenol to inactivate local skin nerve fibers or systematically inactivating peptidergic nerves before sensitization, there was no significant difference in the parameters of the two lateral ears (p 0.05), and capsaicin was used to inactivate local skin nerve fibers before sensitization. There was no statistical difference in each index of the two lateral ears (p0.05). 3. Capsaicin was used 2 days before sensitizing, and was stimulated again 3 days, 6 days, 9 days, 12 days and 15 days after sensitization. There was no significant difference in the indexes of two lateral ears (P 0.05). Conclusion: 1. Capsaicin can prevent the formation of allergic contact dermatitis by blocking the afferent branch (sensitizing stage) and efferent branch (excitation stage) of allergic contact dermatitis in mice. 2. Phenol can block the afferent branches of allergic contact dermatitis in mice. 3. Nervous system plays an important role in the occurrence and development of allergic contact dermatitis in mice.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R758.22

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