淋巴结注射免疫治疗对特应性皮炎患者血清屋尘螨sIgE、IL-4和INF-γ的影响

发布时间：2018-05-12 19:24

本文选题：特异性免疫球蛋白E + 白细胞介素-　；参考：《免疫学杂志》2014年04期

【摘要】：目的探讨淋巴结注射免疫治疗特应性皮炎的分子免疫学机制。方法 72例对屋尘螨过敏的特应性皮炎患者,随机分为治疗组和对照组,每组各36例,对照组给予0.1%糠酸莫米松乳膏、1.2%维生素B6软膏、氯雷他定片、富马酸酮替芬等基础药物治疗,治疗组在上述药物治疗的基础上,超声引导下分别于0周、4周、8周、12周、16周、20周行腹股沟淋巴结内注射屋尘螨过敏原制剂,除0周用药剂量为100 SQ-U外,其余5次剂量均为1 000 SQ-U。治疗前后采用荧光酶联免疫法检测特应性皮炎患者血清屋尘螨sIgE、采用酶联免疫吸附试验(ELISA)方法检测患者血清IL-4和INF-γ值。检测数据应用SPSS19.0统计学软件进行分析。结果 2组中所有患者均完成了20周的治疗。治疗后治疗组血清屋尘螨sIgE和IL-4值由治疗前的(5.06±2.30)KU/L和(16.41±0.91)ng/L分别降至(2.14±1.09)KU/L和(9.52±0.75)ng/L,且均明显低于治疗后对照组(4.63±1.13)KU/L和(16.40±0.92)ng/L,2组比较差异有显著统计学意义(P0.01);治疗组血清INF-γ值由治疗前的(11.34±0.71)ng/L增至(14.78±0.68)ng/L,且明显高于对照组的值(11.32±0.58)ng/L,2组比较有显著性统计学差异(P0.01)。结论屋尘螨过敏原淋巴结注射免疫治疗显著降低患者血清屋尘螨sIgE和IL-4值,同时使血清INF-γ值升高。降低血清sIgE、调节血清IL-4和INF-γ的平衡可能是淋巴结注射免疫治疗的分子免疫机制。
[Abstract]:Objective to investigate the molecular immunological mechanism of lymph node immunotherapy for atopic dermatitis. Methods Seventy-two patients with atopic dermatitis allergic to house dust mite were randomly divided into treatment group (n = 36) and control group (n = 36). The control group was treated with 0.1% momethasone furoate cream and 1.2% vitamin B6 ointment and loratadine tablet. Ketotifen fumarate and other basic drugs were used in the treatment group. On the basis of the above drug treatment, the treatment group was treated with intraperitoneal injection of house dust mite allergen in the inguinal lymph nodes at 0 weeks, 4 weeks, 8 weeks, 12 weeks, 16 weeks and 20 weeks, respectively. Except the dose of 100 SQ-U for 0 weeks, the other 5 doses were 1 000 SQ-U. The serum levels of Siga in patients with atopic dermatitis were detected by fluorescence enzyme-linked immunosorbent assay (Elisa), and serum IL-4 and INF- 纬 values were detected by Elisa. The data were analyzed by SPSS19.0 software. Results all patients in both groups completed 20 weeks of treatment. The levels of sIgE and IL-4 in the treatment group decreased from 5.06 卤2.30)KU/L and 16.41 卤0.91)ng/L before treatment to 2.14 卤1.09)KU/L and 9.52 卤0.75 ng / L, respectively, and were significantly lower than those in the control group (4.63 卤1.13)KU/L and 16.40 卤0.92 ng / L, P 0.01). The value of 11.34 卤0.71)ng/L increased to 14.78 卤0.68 ng / L, and was significantly higher than that of the control group (11.32 卤0.58 ng / L, P < 0.01). Conclusion Immunotherapy with allergen lymph node can significantly decrease the serum sIgE and IL-4 levels and increase the serum INF- 纬 level in patients with pneumoconiosis. Lowering serum Siga E and regulating the balance of serum IL-4 and INF- 纬 may be the molecular immune mechanism of lymph node injection immunotherapy.
【作者单位】：郑州大学第一附属医院皮肤科;中国人民解放军第150中心医院变态反应中心;
【分类号】：R758.2

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