Caspase-14在恶性黑素瘤中的表达及意义

发布时间：2018-05-14 04:25

本文选题：恶性黑素细胞瘤 + caspase-　；参考：《细胞与分子免疫学杂志》2014年11期

【摘要】：目的明确caspase-14在恶性黑素瘤细胞和组织中表达水平及其对肿瘤细胞耐药的影响。方法运用反转录PCR和Western blot技术检测人黑素细胞和恶性黑素瘤细胞内caspase-14的表达。恶性黑素瘤和皮内痣组织中caspase-14表达采用原位杂交和免疫组织化学方法检测。结果 Caspase-14在恶性黑素瘤细胞和黑素细胞中均有表达。T细胞识别的黑素瘤相关抗原1(MART-1)阳性肿瘤细胞中caspase-14表达高于MART-1阴性细胞。Caspase-14表达水平低的肿瘤细胞对化疗药物更敏感(P0.01)。在临床样本中,caspase-14在皮内痣中阳性率为70%,恶性黑素瘤组织中阳性率为97%,差别有统计学意义(P0.05)。结论 Caspase-14在恶性黑素瘤细胞和组织中均有表达。Caspase-14表达水平高的恶性黑素瘤细胞对化疗药物喜树碱和顺铂,以及放射治疗更加抵抗。
[Abstract]:Objective to investigate the expression of caspase-14 in malignant melanoma cells and its effect on drug resistance. Methods reverse transcription PCR and Western blot were used to detect the expression of caspase-14 in human melanocytes and malignant melanoma cells. The expression of caspase-14 in malignant melanoma and intradermal nevus was detected by in situ hybridization and immunohistochemistry. Results the expression of caspase-14 in Caspase-14 positive tumor cells was higher than that in MART-1 negative cells. The tumor cells with low expression level of Caspase-14 were more sensitive to chemotherapeutic drugs. In clinical samples, the positive rate of caspase-14 in intradermal nevus was 70 and that in malignant melanoma was 97. The difference was statistically significant (P 0.05). Conclusion malignant melanoma cells with high expression of Caspase-14 and Caspase-14 are more resistant to chemotherapy drugs camptothecin and cisplatin, as well as radiotherapy.
【作者单位】：四川大学华西基础医学与法医学院病理生理学教研室;四川大学华西基础医学与法医学院法医物证学教研室;
【基金】：国家自然科学基金青年项目(81201560) 四川大学校青年基金(2012SCU11900-7) 四川大学青年教师科研启动基金(2012SCU11020)
【分类号】：R739.5

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