贵州地区麻风与HLA等位基因关联性研究

发布时间：2018-05-15 07:26

本文选题：麻风病 + HLA-A　；参考：《遵义医学院》2017年硕士论文

【摘要】：目的:探讨贵州地区人群HLA-A、HLA-B、HLA-DRBl、HLA-DQ等位基因与麻风病的相关性。方法:收集贵州地区43例成人麻风患者外周静脉血,23例正常健康成人静脉血作为对照组,序列特异性引物聚合酶链反应法(PCR-SSP)进行HLA-A、HLA-B、HLA-DRBl、HLA-DQ等位基因分型。结果:(1)麻风组HLA-A*01,03,26,32等位基因频率低于健康对照组,差异无统计学意义(P均0.05);麻风组HLA-A*11,24,30,31,33,68等位基因频率高于健康对照组,差异无统计学意义(P均0.05)。麻风组HLA-A*02等位基因频率低于健康对照组,差异有统计学意义(x~2=6.629,P=0.013);瘤型麻风组HLA-A*02等位基因频率低于健康对照组,差异有统计学意义(x~2=7.935,P=0.0005);显示HLA-A*02等位基因与麻风病的发病危险度呈负相关。(2)麻风组HLA-B*35,38,40,46,51,52,55等位基因频率低于健康对照组,差异无统计学意义(P均0.05);麻风组HLA-B*08,13,15,27,39,56,58等位基因频率高于健康对照组,差异无统计学意义(P0.05)。瘤型麻风组HLA-B*46等位基因频率低于健康对照组,差异有统计学意义(x~2=3.893,P=0.048),显示HLAB*46等位基因与瘤型麻风的发病危险度呈负相关。(3)麻风组HLA-DRB1*01,08,09,12,13,14,16等位基因频率低于健康对照组,差异无统计学意义(P均0.05);麻风组HLA-DRB1*03,04,11等位基因频率高于健康对照组,差异无统计学意义(P均0.05)。麻风组HLA-DRB1*15等位基因频率高于健康对照组,差异有统计学意义(x~2=3.987,P=0.046)。瘤型麻风组HLA-DRB1*16(P=0.019)等位基因频率低于健康对照组,差异有统计学意义(P0.05);瘤型麻风组HLA-DRB1*15等位基因频率高于健康对照组,差异有统计学意义(x~2=4.840,P=0.028)。显示HLA-DRB1*15等位基因与麻风病的发病危险度呈正相关,HLA-DRB1*16等位基因与瘤型麻风的发病危险度呈负相关。(4)麻风组HLA-DQB1*02,04,05,06等位基因频率低于健康对照组,差异无统计学意义(P均0.05);麻风组HLA-DQB1*03等位基因频率高于健康对照组,差异无统计学意义(P0.05)。瘤型麻风组HLA-DQB1等位基因频率与健康对照组差异无统计学意义(P均0.05)。结核样型麻风组HLA-DQB1等位基因频率与健康对照组差异无统计学意义(P均0.05)。结论:(1)贵州地区麻风组HLA-A*02等位基因对麻风病易感可能存在拮抗作用,可能是贵州地区麻风病的保护基因;HLA-B*46、HLA-DRB1*16等位基因对贵州地区瘤型麻风易感可能存在拮抗作用,可能是贵州地区瘤型麻风的保护基因。(2)贵州地区麻风组HLA-DRB1*15等位基因频率高于健康对照组,瘤型麻风组HLA-DRB1*15等位基因频率高于健康对照组,提示HLA-DRB1*15可能是贵州地区麻风病的易感基因。(3)HLA-DQB1等位基因与贵州地区麻风病的发病危险度无关联。(4)本研究结果与其他国内外麻风病与HLA等位基因的关联性研究结果不尽相同,提示贵州地区人群HLA等位基因与麻风的关联性有一定特殊性。
[Abstract]:Objective: to investigate the association between HLA-DQ alleles and leprosy in the population of Guizhou province. Methods: the peripheral venous blood samples of 43 adult leprosy patients in Guizhou area were collected as control group. The HLA-DQ alleles of HLA-BN HLA-DRBlN were detected by sequence specific primer polymerase chain reaction (PCR-SSPP). Results (1) the allele frequency of alleles in leprosy group was lower than that in the healthy control group (P < 0.05), but the allele frequency of HLA-AM1124A30T31F3O3P68 in leprosy group was higher than that in the healthy control group (P < 0.05), but the allele frequency in leprosy group was lower than that in the control group (P < 0.05), but there was no significant difference between the two groups (P > 0.05). The frequency of HLA-A*02 allele in leprosy group was lower than that in healthy control group (P 0.013), and the frequency of HLA-A*02 allele in leprosy group was lower than that in healthy control group. The allele frequency of HLA-A*02 allele was negatively correlated with the risk of leprosy in leprosy group.) the allele frequency in leprosy group was lower than that in healthy control group. There was no significant difference in allelic frequencies of HLA-B08P08A13152739F5N558 between the leprosy group and the healthy control group (P < 0.05), but there was no significant difference in the allele frequency between the two groups (P < 0.05). The frequency of HLA-B*46 allele in leprosy group was lower than that in healthy control group, and the difference was statistically significant (P < 0.05). The allele frequency of HLAB*46 allele was negatively correlated with the risk of leprosy. There was no significant difference in allele frequencies of HLA-DRB1 / 03P04 / 11 between the leprosy group and the healthy control group (P < 0.05). The frequency of HLA-DRB1*15 allele in leprosy group was higher than that in healthy control group (P 0.046). The allelic frequency of HLA-DRB1A16 (P0. 019) allele in leprosy group was lower than that in healthy control group (P 0. 05), and the frequency of HLA-DRB1*15 allele in leprosy group was higher than that in healthy control group (P 0. 028). There was a positive correlation between HLA-DRB1*15 allele and leprosy risk. There was a negative correlation between HLA-DRB1m16 allele and leprosy risk. The frequency of HLA-DQB1*03 allele in leprosy group was higher than that in healthy control group (P < 0.05). The frequency of HLA-DQB1 allele in leprosy group was not significantly different from that in healthy control group (P < 0.05). The frequency of HLA-DQB1 allele in tuberculous leprosy group was not significantly different from that in healthy control group (P < 0.05). Conclusion the HLA-A*02 allele of leprosy group in Guizhou area may have antagonistic effect on the susceptibility to leprosy, and it may be that the allele of HLA-BN 46 and HLA-DRB1P16 allele may have antagonistic effect on the susceptibility to leprosy in Guizhou area. The frequency of HLA-DRB1*15 allele in leprosy group was higher than that in healthy control group, and the frequency of HLA-DRB1*15 allele in tumor type leprosy group was higher than that in healthy control group. It is suggested that HLA-DRB1*15 may be the susceptible gene of leprosy in Guizhou area. There is no association between HLA-DQB1 allele and leprosy risk of leprosy in Guizhou area.) the results of this study are different from those of other leprosy alleles at home and abroad, and the results of this study are different from those of other leprosy alleles at home and abroad. The results suggest that the association between HLA allele and leprosy in Guizhou population has some particularity.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R755

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