双氢青蒿素对UVB照射小鼠皮肤损伤的光保护作用

发布时间：2018-05-20 08:40

本文选题：双氢青蒿素 + 白介素-　；参考：《中国皮肤性病学杂志》2017年10期

【摘要】：目的研究双氢青蒿素对中波紫外线照射后小鼠皮肤组织白介素(interleukin,IL)-1,IL-6及半胱氨酸天冬氨酸蛋白酶(cysteinylaspartic speahc proteinase)-3,Caspase-8表达的影响,初步探讨青蒿素光保护作用的可能机制。方法 40只BALB/C小鼠随机分为4组,即对照组(C),NB-UVB照射组(UVB),双氢青蒿素组(DHA),双氢青蒿素+NB-UVB组(DHA+UVB)。C组给予生理盐水灌胃,UVB组给予生理盐水灌胃后NB-UVB照射,DHA组给予双氢青蒿素灌胃,DHA+UVB组给予双氢青蒿素灌胃后进行NB-UVB照射。各组灌胃100mg/(kg·d),持续2周。UVB照射剂量为1 J/(cm~2·d),共2周,累积剂量14 J/cm~2,14天后取小鼠背部皮肤组织,采用酶联免疫吸附试验检测各组小鼠皮肤组织IL-1,IL-6水平,RT-PCR,WT-blot检测各组小鼠皮肤组织Caspase-3,Caspase-8变化情况。结果与C组相比,UVB组皮肤组织中IL-1,IL-6明显增加,Caspase-3,Caspase-8 mRNA表达及其蛋白水平均增加,差异均有统计学意义(均P0.05),DHA组小鼠皮肤组织IL-1,IL-6水平没有明显变化;Caspase-3,Caspase-8表达降低,但差异均无统计学意义(均P0.05)。DHA+UVB组与UVB组相比,IL-1,IL-6的浓度增加明显减少,Caspase-3,Caspase-8 mRNA表达及蛋白水平降低,差异均有统计学意义(均P0.05)。结论双氢青蒿素对紫外线所致小鼠光损伤的炎症因子IL-1,IL-6及凋亡蛋白Caspase-3,Caspase-8的产生有一定的抑制作用。提示系统性应用双氢青蒿素对UVB致小鼠光损伤有保护作用。
[Abstract]:Objective to study the effects of dihydroartemisinin on the expression of interleukin-1 (IL-6) and cysteinylaspartic speahc proteinase-3 (Caspase-8) in skin tissue of mice after ultraviolet B irradiation, and to explore the possible mechanism of artemisinin photoprotection. Methods 40 BALB/C mice were randomly divided into 4 groups. The control group was treated with NB-UVB irradiation group, the dihydroartemisinin group with dihydroartemisinin group, the dihydroartemisinin NB-UVB group with dihydroartemisinin UVB).C group with normal saline intragastric perfusion, and the NB-UVB irradiation group with NB-UVB irradiation group with dihydroartemisinin intragastric perfusion of dihydroartemisinin (DHA) group, with dihydroartemisinin group with dihydroartemisinin. NB-UVB irradiation was performed after gastric perfusion. Each group was given intragastric administration of 100mg/(kg dai for 2 weeks. The irradiation dose was 1 J/(cm~2 dai for 2 weeks. The cumulative dose was 14 J / cm ~ (-1) 路min ~ (-1) 路min ~ (-1). The changes of Caspase-3 and Caspase-8 in skin tissue of each group were detected by enzyme linked immunosorbent assay (Elisa). Results compared with group C, IL 1 and IL 6 in UVB group increased significantly the expression of Caspase-3 and Caspase-8 mRNA and the level of Caspase-8 protein. The difference was statistically significant (P 0.05). The level of IL 1 and IL 6 in skin tissue of UVB group was not significantly changed and the expression of Caspase-3 and Caspase-8 was decreased. However, there was no significant difference between P0.05).DHA UVB group and UVB group (P < 0.01). The increase of IL-1and IL-6 level in P0.05).DHA UVB group significantly decreased the expression of Caspase-3, Caspase-8 mRNA and the level of Caspase-8 protein (all P 0.05). Conclusion Dihydroartemisinin has a certain inhibitory effect on the production of the inflammatory cytokines IL-1and IL-6 and the apoptotic protein Caspase-3 Caspase-8 in UV induced light injury in mice. The results suggest that systemic application of dihydroartemisinin can protect mice from UVB induced light injury.
【作者单位】：陕西省人民医院皮肤科;陕西省人民医院中心实验室;陕西省人民医院检验科;西安交通大学第二附属医院皮肤科;
【分类号】：R758.1

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