武汉地区痤疮患者皮损中分离的丙酸杆菌耐药性分析

发布时间：2018-05-26 03:12

本文选题：丙酸杆菌 + 痤疮　；参考：《中国皮肤性病学杂志》2014年02期

【摘要】：目的观察从痤疮患者皮损中分离出的丙酸杆菌对红霉素和四环素的药敏试验结果。方法从痤疮患者皮损中分离丙酸杆菌,厌氧培养获得分离株,经革兰染色、16S rRNA和23S rRNA测序等方法鉴定,采用E-test方法检测分离株对红霉素和四环素的敏感性。结果共分离84株丙酸杆菌,经革兰染色,镜下可见革兰染色阳性的棒状杆菌。所有分离株16S rRNA和23S rRNA扩增序列Blast结果显示:72株与Genbank中痤疮丙酸杆菌(Propionibacterium acnes,P.acnes)16S rRNA和23S rRNA的序列有98%~100%的同源性,12株与Genbank中贪婪丙酸杆菌(Propionibacterium avidum,P.avidum)16S rRNA和23S rRNA的序列有99%~100%的同源性。84株分离株中,所有菌株对四环素敏感,MIC值在0.016~0.50μg/mL间。29株(34.52%)对红霉素耐药,均为高度耐药(MIC256μg/mL);其余55株对红霉素敏感(MIC值0.016~0.38μg/mL)。P.acnes和P.avidum中红霉素耐药株分别为19株(26.39%)、10株(83.33%)。结论武汉地区痤疮患者携带的丙酸杆菌对红霉素耐药率高,对四环素均敏感。该药敏试验结果可指导临床合理应用抗生素。
[Abstract]:Objective to observe the drug sensitivity of Propionibacterium to erythromycin and tetracycline. Methods Propionibacterium propionate was isolated from the skin lesions of acne patients. The isolates were isolated by anaerobic culture. The sensitivity of the isolates to erythromycin and tetracycline was detected by E-test method and identified by 16s rRNA and 23s rRNA sequencing. Results A total of 84 strains of Propionibacterium propionate were isolated. Gram-positive corynebacterium could be detected by Gram staining. All isolates were amplified by 16s rRNA and 23s rRNA. The results of Blast analysis showed that the sequences of 16s rRNA and 23s rRNA of Propionibacterium acnesae in Genbank were 98% 100% homology of 12 strains, and 99% of 100% homology with the sequences of P.avidum16s rRNA and 23s rRNA in Genbank. Sex. 84 isolates, The MIC values of all strains to tetracycline ranged from 0.016 to 0.50 渭 g/mL. 29 strains were resistant to erythromycin, all of them were highly resistant to erythromycin, and the remaining 55 strains to erythromycin sensitive to erythromycin were 0.016 ~ 0.38 渭 g/mL).P.acnes and 19 strains of erythromycin resistance in P.avidum, respectively. Conclusion Propionibacterium propionate carried by acne patients in Wuhan has high resistance to erythromycin and is sensitive to tetracycline. The results of the drug sensitivity test can guide the rational application of antibiotics in clinic.
【作者单位】：武汉市第一医院皮肤科;湖南中医药大学;
【基金】：湖北省感染性皮肤病临床医学研究中心专项基金(2009BCC11)
【分类号】：R758.733

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