HMGB1对角质形成细胞自分泌三种细胞因子的影响

发布时间：2018-05-27 16:39

本文选题：高迁移率蛋白 + 角质形成细胞　；参考：《中国皮肤性病学杂志》2014年08期

【摘要】：目的探讨高迁移率蛋白B1(HMGB1)影响人永生化角质形成细胞(HaCaT)分泌维持黑素细胞生长相关细胞因子的作用。方法 ELISA法检测HMGB1在正常对照组和白癜风患者血清中的表达。免疫荧光和实时定量PCR检测HMGB1及其受体Toll样受体2(TLR2),Toll样受体4(TLR4)和糖基化终产物受体(RAGE)在HaCaT中的表达。ELISA检测白癜风患者血清对于HaCaT分泌碱性成纤维细胞生长因子(bFGF)、干细胞因子(SCF)、内皮素-1(ET-1)的影响。结果相比于对照组,HMGB1在白癜风患者血清中表达升高;HMGB1及其受体在HaCaT中有表达,HMGB1表达在细胞核内;白癜风患者血清培养能够显著降低bFGF,SCF,ET-1的表达。结论角质形成细胞能够在病理状态下(白癜风)释放HMGB1,HMGB1可以降低角质形成细胞分泌的三种细胞因子从而参与白癜风的病理进程。
[Abstract]:Objective to investigate the effect of high mobility protein B1 (HMGB1) on the secretion and maintenance of melanocyte growth related cytokines by human immortalized keratinocytes (HaCaT). Methods ELISA method was used to detect the expression of HMGB1 in the serum of normal control group and vitiligo patients. Expression of HMGB1 and its receptor Toll like receptor 2TLR4TLR4 and glycosylation end product receptor raget in HaCaT. Elisa was used to detect the secretion of basic fibroblast growth factor (bFGFN) to HaCaT in patients with vitiligo by immunofluorescence and real-time quantitative PCR. The effect of cytokine SCF, endothelin-1 (et-1). Results compared with the control group, the expression of HMGB1 in serum of vitiligo patients was higher than that of control group. HMGB1 and its receptors were expressed in HaCaT and the expression of HMGB1 in nucleus was significantly decreased by serum culture of vitiligo patients. Conclusion the release of HMGB1 and HMGB1 by keratinocytes can reduce the secretion of three cytokines by keratinocytes and participate in the pathological process of vitiligo.
【作者单位】：西安交通大学医学院第一附属医院皮肤科;西安交通大学医学院第一附属医院转化医学中心;
【基金】：国家自然科学基金(No.81171504)
【分类号】：R758.41

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