HBD-2、IFN-γ和NF-κBp65在寻常型银屑病皮损中表达的研究

发布时间：2018-06-03 09:09

本文选题：银屑病 + HBD-2　；参考：《山西医科大学》2011年硕士论文

【摘要】：目的:探讨HBD-2、IFN-?、NF-κBp65在寻常型银屑病皮损中的表达和其与银屑病严重程度的关系,以及在寻常型银屑病中的作用机制。方法:采用免疫组化法检测45例银屑病患者皮损组织、非皮损组织及15例正常皮肤组织标本中HBD-2、IFN-?、NF-κBp65的表达,比较不同组别间HBD-2、IFN-?、NF-κBp65的表达的差异并进行相关性分析。根据PASI评分将45例寻常型银屑病患者分为轻、中、重三组,其中轻度组25例,中度组12例,重度组8例。检测轻、中、重皮损处HBD-2、IFN-?、NF-κBp65的表达,比较不同组别间三者表达的差异。结果:1、与正常人皮肤组织和银屑病患者非皮损区相比,银屑病患者皮损区中HBD-2、IFN-?、NF-κBp65的表达明显上调,而非皮损区HBD-2、IFN-?的表达也高于正常人皮肤组织,差异均有统计学意义(P0.05) ;NF-κBp65在皮损区的表达相对于非皮损区、正常皮肤组织显著表达,有统计学意义(P0.05),非皮损区与正常皮肤组织相比差异无统计学意义(P0.05)。2、在寻常型银屑病皮损区HBD-2、IFN-?、NF-κBp65在重度组中的表达高于轻度组和中度组,在中度组的表达高于轻度组,两组之间相比较差异均有统计学意义(P0.05)。3、在寻常型银屑病皮损区IFN-?、NF-κBp65的表达水平呈正相关(r=0.761,P0.05); HBD-2、NF-κBp65的表达水平呈正相关(r=0.740,P0.05);HBD-2、IFN-?的表达水平呈正相关(r=0.851,P0.05)。结论:本研究结果表明HBD-2,IFN-?,NF-κBp65在寻常型银屑病皮损中的表达均升高,且三者之间均存在正相关,我们推测三者之间可能存在相互作用共同参与了银屑病的发病过程。
[Abstract]:Objective: to investigate the expression of NF- 魏 Bp65 in psoriasis vulgaris and its relationship with the severity of psoriasis, and to explore the mechanism of the expression of NF- 魏 Bp65 in psoriasis vulgaris. Methods: the expression of NF- 魏 Bp65 in 45 cases of psoriasis, 15 cases of normal skin and 45 cases of psoriasis were detected by immunohistochemical method. The difference of expression of NF- 魏 Bp65 between different groups was compared and the correlation was analyzed. According to PASI score, 45 patients with psoriasis vulgaris were divided into three groups: mild, moderate and severe, including 25 mild, 12 moderate and 8 severe psoriasis. To detect the expression of NF- 魏 Bp65 in the lesions of light, middle and heavy lesions, and compare the differences among the three groups. Results compared with normal skin tissue and psoriatic non-lesion area, the expression of HBD-2mIFN-tr NF- 魏 Bp65 was up-regulated in psoriatic skin tissue, but not in non-lesion area of psoriasis, and the expression of NF- 魏 Bp65 was significantly up-regulated in psoriatic skin tissue and psoriatic lesions. The expression of NF- 魏 Bp65 in skin lesions was significantly higher than that in normal skin tissues, and the expression of NF- 魏 Bp65 in normal skin tissues was significantly higher than that in normal skin tissues. There was no significant difference between non-skin lesions and normal skin tissues. The expression of HBD-2IFN-fi-NF- 魏 Bp65 in severe psoriasis was higher than that in mild and moderate psoriasis, and the expression of NF- 魏 was higher in moderate and moderate psoriasis than that in mild psoriasis. There was a significant difference between the two groups (P 0.05). The expression of NF- 魏 Bp65 in psoriasis vulgaris was positively correlated with the expression level of NF- 魏 Bp65 in the lesions of psoriasis vulgaris, and the expression level of NF- 魏 Bp65 in HBD-2 was positively correlated with that of P0.05HBD-0.740P0.05in psoriatic psoriasis vulgaris. The expression of NF- 魏 Bp65 was positively correlated with the expression of NF- 魏 Bp65 in psoriasis vulgaris. There was a positive correlation between the expression level and the expression level of P0.05. Conclusion: the results of this study indicate that the expression of NF- 魏 Bp65 in psoriasis vulgaris is increased, and there is a positive correlation among them. We speculate that the interaction among them may be involved in the pathogenesis of psoriasis.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R758.63

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