慢性荨麻疹患者户尘螨sIgE与IL-4的表达及意义

发布时间：2018-06-05 00:24

本文选题：慢性荨麻疹 + IL-4　；参考：《山西医科大学》2015年硕士论文

【摘要】：目的:研究及阐释户尘螨s Ig E与IL-4水平在CU患者血清中的存在情况及相关性。方法:病例组41例,选自2012至2014年期间因躯干、面部、四肢散在蚕豆至鸡蛋大小不等的风团伴瘙痒,且总发病时间反复超过6礼拜,每周发作次数不小于2次,每次发病时皮疹可在1天以内消退不留痕迹,就诊于山大一院皮肤性病科门诊,根据病史及临床表现诊断为慢性荨麻疹,年龄≥12周岁,对以上患者行Allergy Screen免疫印迹法检测后报告单示对户尘螨过敏;健康组35例,有严重心、肝、肾脏疾患的人群以及怀孕期、哺乳期妇女不作为入选对象,所有病例组与健康组人群都没有在30天内使用过激素、免疫调节剂等。户尘螨特异性Ig E水平的测定采用Allergy Screen(敏筛)免疫印迹法,IL-4水平的测定采用ABC-ELISA法,实验所得资料使用SPSS13.0软件进行统计学分析,以t检验比较二组定量资料,以Pearson相关分析比较指标间的相关性,以α等于0.05为检验水准。结果:CU组(慢性荨麻疹患者)血清中的户尘螨s Ig E含量和IL-4指标都显著高表达于健康人群组,二者之间的差异在统计学上具有意义(P0.05);CU患者血清中户尘螨s Ig E与IL-4指标无显著相关性(r为0.171,P0.05)。结论:户尘螨s Ig E、IL-4水平与CU的发病有关;CU病人血清中Ig E存在情况的干扰元素众多。
[Abstract]:Objective: to study and elucidate the existence and correlation of s Ig E and IL-4 levels in serum of patients with CU. Methods: 41 cases were selected from 2012 to 2014 with itching due to wind mass of different sizes of broad beans and eggs, and the total onset time was more than 6 weeks, and the number of episodes per week was not less than 2 times. Each time the rash disappeared within one day without leaving any trace. It was diagnosed as chronic urticaria according to its history and clinical manifestations, aged more than 12 years, at the Department of Dermatology and venereal Diseases, first Hospital of Shandong University. The Allergy Screen Western blotting test showed that the above patients were allergic to dust mites, 35 healthy people with severe heart, liver and kidney diseases, and pregnant and lactating women were not included in the study. No hormone or immunomodulator was used within 30 days in all patients and healthy subjects. The specific IgE level of dust mites was determined by Allergy screen Western blotting method and IL-4 level was determined by ABC-ELISA method. The experimental data were statistically analyzed by SPSS13.0 software, and the quantitative data of two groups were compared with t test. Using Pearson correlation analysis to compare the correlation between the indicators, 伪 = 0. 05 as the test level. Results the s Ig E content and IL-4 index of dust mites in the serum of the patients with chronic urticaria were significantly higher than those in the control group. There was no significant correlation between s Ig E and IL-4 in patients with P0.05 or CU. Conclusion: the level of sig Ehl 4 is related to the occurrence of CU. There are many interfering elements of IgE in serum of patients with CU.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R758.24

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