基于THP-1细胞的皮肤致敏体外检测方法

发布时间：2018-06-07 21:21

本文选题：皮肤致敏 + THP-细胞　；参考：《中国比较医学杂志》2017年04期

【摘要】：目的建立基于人细胞系的替代皮肤致敏动物实验的体外检测方法(h-CLAT),并对化学品、日用化学产品和化妆品植物原料进行皮肤致敏性检测。方法体外培养人急性单核细胞白血病细胞(THP-1),不同浓度受试物与细胞共孵育24 h,通过流式细胞仪检测细胞表面标志物CD86和CD54活化的差异,并对11种已知皮肤致敏化学物质和9种未知样品进行皮肤致敏性预测。同时对未知样品进行豚鼠局部封闭涂皮法验证。结果使用h-CLAT方法准确区分了11种参考物质的皮肤致敏性,9种受试样品中,7种样品被判断为阴性皮肤致敏物质,2种植物提取物被确认为皮肤致敏疑似物质。9种样品的预测结果与动物实验一致。结论 h-CLAT体外检测方法可以代替部分动物测试,用于可溶性皮肤致敏物质的预测。
[Abstract]:Objective to establish an in vitro method for the detection of human cell lines in substitute skin sensitizing animals, and to detect the skin sensitivities of chemicals, daily chemical products and cosmetic plant materials. Methods Human acute monocytic leukemia cells were cultured in vitro and incubated with different concentrations for 24 h. The activation of CD86 and CD54 was detected by flow cytometry. Eleven known skin sensitizing chemicals and 9 unknown samples were predicted for skin sensitization. At the same time, the unknown samples were verified by the method of local seal coating in guinea pigs. Results the h-CLAT method was used to accurately distinguish the skin sensitivities of 11 reference substances. 7 samples were judged as negative skin sensitizers and 2 plant extracts were identified as suspected skin sensitizers. 9 samples were identified as skin sensitized suspected substances. The predicted results are consistent with animal experiments. Conclusion in vitro detection of h-CLAT can be used to predict soluble skin sensitizers instead of partial animal tests.
【作者单位】：广东出入境检验检疫局技术中心;广东药科大学;广州市华代生物科技有限公司;
【基金】：广东省科技计划项目(2015A030402005)
【分类号】：R758.2

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