PM2.5对人皮肤角质形成细胞自噬水平的影响

发布时间：2018-06-08 04:45

  本文选题：PM. + 角质形成细胞　； 参考：《中国皮肤性病学杂志》2017年07期

【摘要】：目的研究空气细颗粒物(PM2.5)对人皮肤角质形成细胞自噬水平的影响。方法收集武汉市区空气中的PM2.5,分离并提取其水溶性及非水溶性成分,以不同的终浓度处理人永生化角质形成细胞(HaCaT细胞)。利用细胞免疫荧光染色和Western印迹法检测人角质形成细胞中自噬相关蛋白LC3的表达。结果细胞免疫荧光结果表明,经PM2.5染毒液诱导的细胞LC3表达量均高于对照组,且呈浓度依赖性升高。Western印迹检测结果显示,实验组细胞LC3-II/I比值明显高于对照组(P0.05,除B1组);相同浓度下,PM2.5水溶性成分与非水溶性成分比较作用强度差异无统计学意义(P0.05)。结论 PM2.5可诱导人皮肤角质形成细胞发生自噬,并与浓度有明显相关性,其诱导的机制可能是细胞对抗氧化应激损伤及细胞凋亡的一种自我保护反应。
[Abstract]:Objective to study the effect of PM2.5) on autophagy of human skin keratinocytes. Methods PM2.5 was collected from air of Wuhan urban area and its water-soluble and insoluble components were isolated and extracted. HaCaT cells were treated with different final concentrations of human immortalized keratinocytes. The expression of autophagy associated protein LC3 in human keratinocytes was detected by immunofluorescence staining and Western blot. Results the results of cellular immunofluorescence showed that the level of LC3 expression induced by PM2.5 was higher than that in control group, and the expression of LC3 was increased in a concentration-dependent manner. Western blot analysis showed that the expression of LC3 was increased in a concentration dependent manner. The ratio of LC3-II-1 / I in the experimental group was significantly higher than that in the control group (P 0.05, except group B1), and there was no significant difference in the action intensity between the water-soluble component of PM2.5 and the non-water-soluble component at the same concentration. Conclusion PM2.5 can induce autophagy of human skin keratinocytes and has a significant correlation with the concentration. The mechanism of PM2.5 may be a self-protective response of cells to antioxidant stress injury and apoptosis.
【作者单位】： 武汉大学附属中南医院皮肤性病科;
【分类号】：R751
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本文编号：1994535