白介素-22在扁平苔藓皮损中的表达及意义

发布时间：2018-06-09 00:13

本文选题：扁平苔藓 + IL-22　；参考：《吉林大学》2017年硕士论文

【摘要】：扁平苔藓是一种临床上比较常见的累及皮肤黏膜的瘙痒性疾病,典型皮损为紫罗兰色的扁平的多角形丘疹,表面有蜡样薄膜,伴有少许鳞屑;典型粘膜表现为Wickham纹。扁平苔藓病因学机制目前尚未完全明确,但基底细胞液化变性和真皮浅层淋巴细胞带状浸润的病理现象提示细胞介导的免疫反应与该病的发病机制密切相关。IL-22是一种由Th17和Th22为主的免疫细胞分泌产生的新型CD4+Th细胞因子,主要生物学功能为微生物防御、保护和修复组织损伤及急性期反应,IL-22独特的免疫介导形式使其在很多炎症性、自身免疫性、肿瘤性皮肤病中发挥重要作用。目前对IL-22在扁平苔藓中的研究较少,因此本实验通过检测IL-22在扁平苔藓皮损中的表达情况,并与正常皮肤相比较,以推测IL-22在扁平苔藓发病过程中可能起的作用,为扁平苔藓的诊断和治疗开辟新的思路。目的:探讨白介素-22在扁平苔藓皮损中的表达情况,为深入揭露疾病的发病机制提供理论基础,为临床治疗提供新的思路和靶点。方法:实验组30例(男18例,女12例,年龄19~63岁,平均年龄42.3±8.4岁),均为吉林大学第二医院皮肤科经临床和病理检查确诊的扁平苔藓患者的病变皮肤;对照组10例(男5例,女5例,年龄22-66岁,平均年龄45.6±7.3岁),均为吉林大学第二医院皮肤外科手术切除的正常皮肤。两组患者年龄和性别相匹配。采用免疫组织化学方法检测IL-22在扁平苔藓皮损和正常皮肤组织中的表达情况,并运用统计学软件进行结果分析。结果:在30例实验组中,16例为强阳性,8例为阳性,4例为弱阳性,2例为阴性。IL-22主要表达于扁平苔藓表皮全层的角质形成细胞和真皮浅层浸润的淋巴细胞胞质中,呈棕黄色颗粒。在10例对照组中,1例为强阳性,2例为阳性,4例为弱阳性,3例为阴性。IL-22主要表达于正常皮肤组织基底层附近的角质形成细胞中,呈淡黄色颗粒。其中IL-22在扁平苔藓皮损中的表达水平明显高于在正常皮肤组织中的表达水平,差异具有显著统计学意义(P0.01)。结论:1、IL-22在大多数扁平苔藓皮损的表皮全层呈阳性表达,并且IL-22在扁平苔藓表皮中的表达水平明显高于正常皮肤组织,提示它可能参与扁平苔藓的发病。2、IL-22在大部分扁平苔藓真皮浅层淋巴细胞浸润区呈阳性表达,提示它可能是造成扁平苔藓炎症细胞浸润的原因之一。3、IL-22在大多数正常皮肤组织中呈弱阳性表达,主要定位于表皮的基底层,提示它可能参与角质形成细胞的正常分化。
[Abstract]:Lichen planus is a common pruritus disease involving skin and mucosa. Typical lesions are violet-colored flat polygonal papules with waxy membrane with a few scales and typical mucosal appearance is Wickham's stria. The etiological mechanism of lichen planus is not completely clear. However, the pathological phenomena of liquefaction of basal cells and band infiltration of superficial dermal lymphocytes suggest that cell-mediated immune response is closely related to the pathogenesis of the disease. IL-22 is produced by the secretion of Th17 and Th22 mainly by immune cells. Novel CD4 Th cytokines, The main biological functions are microbial defense, protection and repair of tissue damage and the unique immune-mediated form of IL-22, which play an important role in many inflammatory, autoimmune and tumorous skin diseases. There are few studies on the expression of IL-22 in lichen planus (LP), so the expression of IL-22 in Lichen planus was detected and compared with that in normal skin to speculate the possible role of IL-22 in the pathogenesis of Lichen planus. It provides a new idea for the diagnosis and treatment of lichen planus. Objective: to investigate the expression of interleukin-22 in the lesions of lichen planus (Lichen planus), to provide a theoretical basis for revealing the pathogenesis of the disease, and to provide new ideas and targets for clinical treatment. Methods: 30 cases (male 18, female 12, age 1963 years old, mean age 42.3 卤8.4 years) in the experimental group were all skin lesions of lichen planus patients confirmed by clinical and pathological examination in the Department of Dermatology, second Hospital of Jilin University, and 10 cases (5 males) in the control group. Five women, aged 22-66 years, with an average age of 45.6 卤7.3 years, were all normal skin surgically resected by skin surgery in the second Hospital of Jilin University. Age and sex were matched between the two groups. Immunohistochemical method was used to detect the expression of IL-22 in lichen planus lesions and normal skin tissues, and the results were analyzed by statistical software. Results: in the 30 cases of experimental group, 16 cases were strongly positive and 8 cases were positive, 4 cases were weak positive, 2 cases were negative. IL-22 was mainly expressed in keratinocytes of the whole layer of lichen planus epidermis and the cytoplasm of lymphocytes infiltrated in the superficial dermis, showing brown granules. In 10 cases of control group, 1 case was strongly positive, 2 cases were positive, 4 cases were weak positive, 3 cases were negative. IL-22 was mainly expressed in keratinocytes near the basal layer of normal skin tissue. The expression of IL-22 in lichen planus lesions was significantly higher than that in normal skin tissues, and the difference was statistically significant (P 0.01). Conclusion the expression of IL-22 in the epidermis of most lichen planus lesions was positive, and the expression level of IL-22 in the epidermis of lichen planus was significantly higher than that in normal skin tissue. It suggests that it may be involved in the pathogenesis of lichen planus. 2IL-22 is positively expressed in the infiltrating area of most of the epidermis lymphocytes of lichen planus. These results suggest that it may be one of the causes of Lichen planus inflammatory cell infiltration. IL-22 is weakly expressed in most normal skin tissues, mainly located in the basal layer of epidermis, suggesting that IL-22 may be involved in the normal differentiation of keratinocytes.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R758.65

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