Smad蛋白及其mRNA在病理性瘢痕上皮和瘢痕癌中的表达及意义

发布时间：2018-06-15 21:08

本文选题：瘢痕癌 + 病理性瘢痕组织　；参考：《中国老年学杂志》2017年12期

【摘要】：目的分析smad与病理性瘢痕上皮和瘢痕癌的相关性。方法采用免疫组织化学(SP法)及实时荧光定量聚合酶链反应分别对病理性瘢痕组织标本15份、瘢痕癌组织标本15份,正常皮肤组织标本20份进行研究,利用图像分析软件,计算三组标本平均观密度、阳性面积以及2-ΔΔCT数值的表达。结果 (1)smad的蛋白及mRNA在正常皮肤组织标本及病理性瘢痕组织标本中呈现阴性及弱阳性,在瘢痕癌组织标本呈现强阳性表达。(2)瘢痕癌组织标本表达水平(阳性面积)、表达强度差异有统计学意义(P0.05),但是正常皮肤组织标本及病理性瘢痕组织标本之间不存在差异(P0.05)。(3)smad的蛋白及mRNA表达呈正相关关系(r=9.34 P0.05)。结论 smad与瘢痕癌的表达有关联性,可作为瘢痕癌靶向治疗的研究重点。
[Abstract]:Objective to analyze the correlation between smad and pathological scar epithelium and scar carcinoma. Methods Fifteen specimens of pathological scar tissue, 15 specimens of scar carcinoma and 20 specimens of normal skin were studied by immunohistochemistry and real-time fluorescence quantitative polymerase chain reaction. Image analysis software was used. The mean apparent density, positive area and the expression of 2-螖 CT were calculated. Results the protein and mRNA of SMA were negative and weakly positive in normal skin tissue and pathological scar tissue. The expression level of scar carcinoma tissue (positive area, expression intensity difference is statistically significant (P 0.05), but there is no difference between normal skin tissue and pathological scar tissue specimen.) the expression level of scar cancer tissue is strongly positive. 2) the expression level of scar carcinoma tissue is significantly different from that of normal skin tissue and pathological scar tissue (P 0.05). There was a positive correlation between the protein expression and mRNA expression of P0.05, P0.05, P0.05, and the expression of protein and mRNA was positively correlated with that of 9.34 P0.05. Conclusion smad is associated with the expression of cicatricial carcinoma.
【作者单位】：吉林省人民医院烧伤整形外科;吉林油田总医院烧伤科;
【基金】：吉林省自然科学基金项目(201215206)
【分类号】：R739.5

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