咪喹莫特诱导的银屑病鼠模型皮损STAT3表达及活化STAT3水平

发布时间：2018-06-19 22:11

本文选题：STAT + p-STAT　；参考：《中国皮肤性病学杂志》2017年06期

【摘要】：目的探讨咪喹莫特诱导的银屑病鼠(IIPM)模型皮损角质形成细胞(KCs)STAT3的表达和活化STAT3(p-STAT3)的水平及其意义。方法采用免疫组织化学Polymer酶标二抗法,检测16例IIPM皮损和16例取材于对照鼠正常皮肤组织KCs中STAT3的表达和p-STAT3的水平,并利用PASI对IIPM皮损进行临床积分(TCS)评价。结果 IIPM皮损KCs中STAT3表达显著高于对照鼠正常皮肤组织(后者呈低表达)(P=0.018),且其p-STAT3水平亦显著高于后者(后者呈低水平)(P=0.003);IIPM皮损KCs STAT3表达、p-STAT3水平均与IIPM皮损TCS之间存在正相关(rs=0.58,0.76;P=0.019,0.001),随着STAT3表达的增加、p-STAT3水平的升高,皮损TCS也呈升高趋势。结论 KCs STAT3的表达增加和活化对IIPM模型银屑病样皮肤炎症性皮损的形成可能起重要作用,可利用IIPM模型进行关于STAT3的研究。
[Abstract]:Objective to investigate the expression of KCsMS-STAT3 and the level of activated STAT3p-STAT3 in IIPM model of psoriatic rats induced by IIPM. Methods the expression of STAT3 and the level of p-STAT3 in KCs of 16 cases of IIPM skin lesions and 16 cases of normal skin tissues were detected by immunohistochemical Polymer enzyme-labeled antibody method, and the clinical integral TCSs were evaluated by PASI in IIPM skin lesions. Results the expression of STAT3 in KCs of IIPM lesions was significantly higher than that in normal skin tissues of control rats (the latter showed a low expression level of P0. 018, and the level of p-STAT3 was also significantly higher than that of the latter. The latter presented a low level of expression of KCs STAT3 in the lesions of IIPM and the expression of p-STAT3 in the lesions of IIPM and TCS of IIPM lesions. In the positive correlation, the level of p-STAT3 was increased with the increase of STAT3 expression, and the expression of STAT3 was increased with the increase of the expression of STAT3, and the level of p-STAT3 was increased with the increase of the expression of STAT3. TCS of lesions also showed an increasing trend. Conclusion the increased expression and activation of KCs STAT3 may play an important role in the formation of psoriatic inflammatory skin lesions in IIPM model.
【作者单位】：首都医科大学附属北京朝阳医院西区皮肤科;首都医科大学附属北京朝阳医院皮肤科;西安交通大学第二附属医院皮肤科;
【基金】：国家自然科学基金(81441126)
【分类号】：R-332;R758.63

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