大蒜素对皮肤基底细胞癌细胞放射治疗敏感性的影响及其与自噬水平的关系

发布时间：2018-06-21 01:11

本文选题：皮肤基底细胞癌 + A细胞　；参考：《中国皮肤性病学杂志》2017年06期

【摘要】：目的探讨大蒜素(Allicin)对皮肤基底细胞癌细胞放射治疗敏感性的影响及其与自噬水平的关系。方法采用MTT法检测Allicin对A431细胞的生长抑制作用,记录半数抑制浓度(IC50);将细胞分为4组:照射组(IR组)、Allicin组、IR+Allicin组以及对照组。细胞自噬水平的检测采用流式细胞仪。结果MTT检测结果显示,A431细胞经Allicin处理24,48,72h后,其活性受到显著的抑制,抑制程度受到时间及浓度的影响。同一浓度下,不同时间比较以及同一时间内不同浓度的比较,A431细胞活性的差异均具有统计学意义(P均0.05)。Allicin作用于A431细胞24,48,72h的IC50分别为34.14,17.78,6.72mmol/L,差异均具有统计学意义(P均0.05)。IR+Allicin组的SF_2,D_0,D_q分别为:75.81,6.46,1.34;IR组的SF_2,D_0,D_q分别为96.21,8.60,2.85。在各照射剂量点A431细胞的SF均显著低于IR组。与IR组比较,IR+Allicin组的肩区逐渐缩小,说明细胞的损伤修复能力呈现降低趋势。Allicin组和IR+Allicin组的A431细胞自噬水平均高于对照组,差异有统计学意义(P均0.05)。结论 Allicin能上调人皮肤基底细胞癌A431细胞的自噬水平,进而增加A431细胞的放疗敏感性。
[Abstract]:Objective to investigate the effect of allicin on radiosensitivity of skin basal cell carcinoma (BCC) and its relationship with autophagy level. Methods the growth inhibitory effect of Allicin on A431 cells was measured by MTT assay, and the IC50 inhibitory concentration was recorded. The cells were divided into 4 groups: irradiation group (IR group), Allicin group (group C) and control group (group C). The level of autophagy was measured by flow cytometry. Results the results showed that the activity of A431 cells was significantly inhibited after treated with Allicin for 72 h, and the inhibition degree was influenced by time and concentration. At the same concentration, There were significant differences in the activity of A431 cells between different time groups and different concentrations at the same time. The IC50 of A431 cells treated with 0.05).Allicin for 72 h was 34.14, 17.787, 6.72mmol / L, respectively, and the difference was statistically significant. The difference was statistically significant in the SF2D0DQ scores of Allicin group. The number of S / S / D / D / D in the IR group is 96.21 / 8.60 / 2.85 respectively. SF of A431 cells was significantly lower than that of IR group. Compared with IR group, the shoulder area of IR Allicin group was gradually reduced, indicating that the ability of cell damage and repair showed a decreasing trend. The autophagy level of A431 cells in the Allicin group and IR Allicin group was higher than that in the control group, and the difference was statistically significant (P 0.05). Conclusion Allicin can upregulate the autophagy level of human basal cell carcinoma A 431 cells and then increase the radiosensitivity of A431 cells.
【作者单位】：河南省商丘市第一人民医院;
【分类号】：R739.5

【参考文献】