IL-36α对银屑病小鼠皮肤病变的影响及其作用机制

发布时间：2018-06-27 02:51

本文选题：银屑病 + 白细胞介素α　；参考：《山东医药》2017年28期

【摘要】：目的探讨白细胞介素36α(IL-36α)对银屑病小鼠皮肤病变的影响及其作用机制。方法将30只雌性小鼠随机分为对照组、模型组及观察组,每组10只。模型组及观察组于背部裸露皮肤处涂抹5%咪喹莫特乳膏62.5 mg,对照组涂抹等量凡士林乳膏,1次/d,连续8天。观察组隔日皮下注射IL-36α5μg,对照组、模型组注射等量PBS,共注射4次。评估各组建模第1~8天银屑病皮损面积和严重程度评分(PASI评分)。建模第8天处死,测量表皮垂直厚度,分别采用实时荧光定量PCR法、Western blotting法检测皮肤组织血管内皮生长因子(VEGF)和IL-36αmRNA和蛋白相对表达量。结果随建模时间延长,模型组和观察组PASI评分逐渐升高(P均0.05)。观察组、模型组建模第5天开始PASI评分明显升高,以观察组升高更明显(P均0.05)。观察组表皮垂直厚度为(98.519±3.442)μm,模型组为(68.383±2.499)μm,对照组为(9.804±1.682)μm,组间两两比较P均0.05。观察组、模型组和对照组皮肤组织VEGF、IL-36αmRNA和蛋白相对表达量依次降低,组间两两比较P均0.05。结论IL-36α可加重银屑病小鼠皮肤病变,其机制可能与促进VEGF表达有关。
[Abstract]:Objective to investigate the effect and mechanism of interleukin 36 伪 (IL 36 伪) on psoriasis in mice. Methods 30 female mice were randomly divided into control group, model group and observation group with 10 mice in each group. The model group and the observation group were smeared with 5% Imiquimod cream 62.5 mg on the bare skin of the back, while the control group was smeared with the same amount of vaseline cream once a day for 8 days. IL-36 伪 5 渭 g was injected subcutaneously every other day in the observation group, and PBS4 times in the control group and the model group. The psoriasis lesion area and severity score (PASI score) were evaluated on the 1st day of modeling in each group. On the 8th day of modeling, the vertical thickness of epidermis was measured, and the relative expressions of vascular endothelial growth factor (VEGF) and IL-36 伪 mRNA and protein in skin tissue were detected by real-time fluorescence quantitative PCR and Western blotting. Results the PASI score of model group and observation group increased gradually with the prolongation of modeling time (P 0.05). In the observation group, the PASI score of the model group was significantly higher than that of the observation group (P 0.05). The vertical thickness of epidermis was (98.519 卤3.442) 渭 m in the observation group, (68.383 卤2.499) 渭 m in the model group and (9.804 卤1.682) 渭 m in the control group. The relative expression of VEGF IL-36 伪 mRNA and protein in the skin tissues of the observation group, the model group and the control group decreased in turn, with a significant difference between the two groups (P 0.05). Conclusion IL-36 伪 can aggravate skin lesions in psoriatic mice, and its mechanism may be related to the promotion of VEGF expression.
【作者单位】：南方医科大学附属广东省第二人民医院;南方医科大学第三临床医学院;
【基金】：广东省中医药局基金资助项目(20141008)
【分类号】：R758.63

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