Tempol对UVB所致无毛鼠皮肤光老化的防护作用

发布时间：2018-07-13 18:53

【摘要】：目的:评价Tempol对UVB所致无毛鼠皮肤光老化的防护作用。方法:72只无毛鼠分为正常对照组(A组)、UVB照射对照组(B组)、外用0.36% Tempol 1 h后照射UVB组(C组)、外用0.36%Tempol 2 h后照射UVB组(D组)、外用0.36% Tempol 4 h后照射UVB组(E组)和外用0.36% Tempol 8 h后照射UVB组(F组)。末次UVB照射后取其背部皮肤,在显微镜下观察真皮内胶原纤维和弹力纤维含量的变化及病理形态学改变。以生化分析方法测定丙二醛(MDA)及羟脯氨酸(HYP)含量。结果:B、C、E和F组真皮胶原纤维及弹力纤维均增粗、断裂、排列紊乱,皮肤丙二醛含量均显著升高,皮肤羟脯氨酸含量均显著下降,与A和D组比较差异有统计学意义(P0.01)。结论:外用0.36% Tempol对UVB所致无毛鼠皮肤光老化具有防护作用,有效防护时段可能在用药后1～4 h。
[Abstract]:Objective: to evaluate the protective effect of Tempol on photoaging of hairless rat skin induced by UVB. Methods Seventy-two hairless rats were divided into normal control group (group A) and UVB irradiation control group (group B). UVB group (group C) was treated with 0.36% Tempol for 1 h, UVB group (group D) was irradiated with 0.36% Tempol for 2 h, UVB group (group E) was irradiated with 0.36% Tempol for 4 h and UVB group (group E) was treated with 0.36% Tempol for 4 hours. UVB group (group F) was irradiated by Tempol for 8 h. After the last UVB irradiation, the contents of collagen and elastic fibers in the dermis and the histopathological changes were observed under microscope. The contents of malondialdehyde (MDA) and hydroxyproline (HYP) were determined by biochemical analysis. Results the dermal collagen fibers and elastic fibers were thicker, ruptured, disordered, and the content of malondialdehyde and hydroxyproline were significantly increased in group E and F, and there was significant difference between group A and group D (P0.01). Conclusion: topical use of 0.36% Tempol has protective effect on photoaging induced by UVB in hairless mice, and the effective protective period may be 1 hour after treatment.
【作者单位】：青岛大学医学院附属医院皮肤科;烟台毓璜顶医院皮肤科;北京大学医学部实验动物科学部;
【基金】：山东省自然科学基金资助课题(编号:Y20070024)
【分类号】：R751

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