核壳型壳聚糖纳米纤维的制备及其在黑素细胞移植中的应用

发布时间：2018-07-16 09:22

【摘要】：白癜风是一种常见多发的色素脱失性皮肤病,我国发病率为0.56%。尽管不直接造成身体伤害,白癜风能严重影响患者的生活质量。黑素细胞悬液移植是近年来治疗白癜风的热门疗法,针对治疗过程中存在的活动部位细胞移植难以固定和生长环境的改变对移植细胞的损伤的难题,运用组织工程技术构建支持黑素细胞贴附、生长和增殖的组织工程膜,能有效地解决上述问题,从而改善治疗效果。为此,本课题选用同轴电纺技术,以醋酸为溶剂、PEO为核层、壳聚糖为壳层,制备核壳型壳聚糖纳米纤维膜,详细研究工艺条件对纤维成型及形貌的影响,并考察了戊二醛交联对纤维膜性能及稳定性的影响,用纳米纤维膜培养黑素细胞,研究细胞的贴附、生长和增殖情况。研究表明所制备的纳米纤维膜存在与设计相一致的核壳型纳米纤维;用戊二醛蒸汽对纳米纤维膜进行交联,提高了纳米纤维膜的泡水质量稳定性和吸水率,同时提高膜的力学性能;该纳米纤维膜能支持黑素细胞的贴附、生长和增殖,其上的黑素细胞形态和功能表达正常,表明这种纳米纤维膜可以作为黑素细胞组织工程膜。本工作的创新点有:利用同轴电纺技术制备了核壳型聚环氧乙烷-壳聚糖纳米纤维膜,通过戊二醛交联可提高纤维膜的力学性能和耐水稳定性,纤维膜具有良好的生物相容性,能用作黑素细胞组织工程膜。
[Abstract]:Vitiligo is a common and frequent dermatosis with pigment loss. The incidence of vitiligo is 0.56 in China. Vitiligo can seriously affect the quality of life of patients, although it does not directly cause physical damage. Melanocyte suspension transplantation is a popular therapy for vitiligo in recent years. Tissue engineering technology is used to construct tissue engineering membrane supporting melanocyte adhesion, growth and proliferation, which can effectively solve the above problems and improve the therapeutic effect. In this paper, coaxial electrospinning technology was used to prepare core-shell chitosan nanofiber membrane with acetic acid as the solvent and chitosan as the shell layer. The effects of process conditions on the shaping and morphology of the fiber were studied in detail. The effects of glutaraldehyde crosslinking on the properties and stability of fiber membrane were investigated. Melanocytes were cultured with nano-fiber membrane to study the adhesion, growth and proliferation of the cells. The results show that there are core-shell nanofibers which are consistent with the design, and the crosslinking of nanofiber membranes by glutaraldehyde vapor can improve the water quality stability and water absorption of the nanofibers. The nano-fiber membrane can support the adhesion, growth and proliferation of melanocytes, and the morphology and function of melanocytes are normal, indicating that the nano-fiber membrane can be used as melanocyte tissue engineering membrane. The innovations of this work are as follows: Core-shell poly (ethylene oxide) chitosan nanofiber membranes were prepared by coaxial electrospinning. The mechanical properties and water resistance of the membranes were improved by glutaraldehyde crosslinking, and the fiber membranes had good biocompatibility. It can be used as melanocyte tissue engineering membrane.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R758.41;TQ340.64

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