优可洛外用治疗尖锐湿疣的机理初探

发布时间：2018-07-18 09:49

【摘要】：背景：尖锐湿疣(CA)是一种常见的性传播疾病之一,近年来发病率不断上升,再加上其能诱发宫颈癌、鳞状上皮细胞癌等恶性肿瘤,故日益受到人们的重视。目前临床上治疗尖锐湿疣的中药外用药物很多,但大都作用机制不明。优可洛是我科近年来治疗尖锐湿疣使用的中药提取物外用药,疗效显著,本课题将从细胞生物学方面对优可洛的作用机制进行探讨。方法：采用免疫组化SP法检测优可治疗前后17例女性尖锐湿疣患者的皮损局部的Mcm2和Caspase-3的表达情况,并用TUNEL法检测治疗前后表皮细胞的凋亡情况。应用SPSS17.0统计软件分析。结果： 17例女性尖锐湿疣患者治疗前局部皮损的细胞增殖指数为55.35±14.41,治疗后局部皮损的细胞增殖指数为16.06±14.10,差异有显著统计学意义(z=-3.623aP0.01)；治疗前Caspase-3的表达为6.12±2.93,治疗后Caspase-3的表达为9.59±6.84,差异有显著统计学意义(t=2.368,P0.05)；治疗前TUNEL检测的细胞凋亡指数为18.12±9.77,治疗后为27.82±15.19,差异有统计学意义(z=-1.967aP0.05)。结论：优可洛的作用机制,可能是通过抑制表皮细胞增殖,促进细胞凋亡而发挥作用的。
[Abstract]:Background: condyloma acuminatum (CA) is one of the most common sexually transmitted diseases. At present, there are a lot of external drugs for treating condyloma acuminatum, but the mechanism of most of them is unclear. Yucoluo is a traditional Chinese medicine extract used in recent years to treat condyloma acuminatum, the curative effect is remarkable, this subject will discuss the action mechanism of Yucolu from the aspect of cell biology. Methods: the expression of Mcm2 and Caspase-3 in the lesions of 17 female condyloma acuminatum patients before and after treatment were detected by immunohistochemical SP method, and the apoptosis of epidermal cells was detected by Tunel method. SPSS 17.0 statistical software was used to analyze. Results: the proliferative index of local skin lesions was 55.35 卤14.41 in 17 female patients with condyloma acuminatum before treatment, and 16.06 卤14.10 in local lesions after treatment. The expression of Caspase-3 was 6.12 卤2.93 before treatment and 9.59 卤6.84 after treatment, the difference was statistically significant (t 2.368 P 0.05), and the apoptotic index was 18.12 卤9.77 before treatment and 27.82 卤15.19 after treatment (z-1.967aP0.05). Conclusion: the mechanism of euconolol may play a role by inhibiting the proliferation of epidermal cells and promoting cell apoptosis.
【学位授予单位】：广州中医药大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R752.53

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