Kaposi肉瘤组织中人类疱疹病毒8型的免疫组化检测

发布时间：2018-08-02 16:03

【摘要】：目的：人类疱疹病毒8型在自然人群特别是新疆少数民族中有较高的感染率，探讨免疫组织化学方法检测Kaposi肉瘤中人类疱疹病毒8型的可行性及其诊断意义。方法：采用免疫组织化学技术分别检测58例Kaposi肉瘤组织斑片期、结节期和40例化脓性肉芽肿组织中HHV-8的表达，检测结果采用卡方检验四格表Fisher确切概率法分析Kaposi肉瘤组织斑片期、结节期及化脓性肉芽肿组织中HHV-8表达的差异。结果：1.HHV-8在Kaposi肉瘤真皮瘤体中的表达阳性率为94.83%(55/58)，在化脓性肉芽肿中的表达均为阴性，在Kaposi肉瘤组织和血管瘤组织瘤体中表达在统计学有显著性差异（P＜0.05）。2.HHV-8在Kaposi肉瘤组织表皮中表达阳性率为6.90%（4/58），血管内皮细胞中的表达阳性率为91.38%(53/58)，真皮梭形细胞中表达阳性率94.83%(55/58)。3.结节期Kaposi肉瘤中表达HHV-8阳性的细胞所占比例较斑片期及斑块期明显增多，，其差异有统计学意义（P0.05）。结论：免疫组织化学方法检测人类疱疹病毒8型在Kaposi肉瘤的诊断尤其是鉴别诊断中具有重要意义。
[Abstract]:Objective: human herpesvirus 8 has a high infection rate in natural population, especially in Xinjiang ethnic minorities. To explore the feasibility and diagnostic significance of immunohistochemical method for detection of human herpesvirus 8 in Kaposi sarcoma. Methods: immunohistochemical technique was used to detect the expression of HHV-8 in 58 cases of Kaposi's sarcoma at macular stage, nodular stage and 40 cases of suppurative granuloma. Results the difference of HHV-8 expression in Kaposi sarcoma tissue in macular stage, nodular stage and suppurative granuloma was analyzed by chi-square test four grid table Fisher exact probability method. Results 1. The positive rate of HHV-8 was 94.83% (55 / 58) in Kaposi sarcoma dermis, and negative in suppurative granuloma. The positive rate of HHV-8 expression was 6.90% (4 / 58) in epidermis of Kaposi sarcoma, 91.38% (53 / 58) in vascular endothelial cells and 94.83% (55 / 58) in dermal fusiform cells. The proportion of HHV-8 positive cells in nodular Kaposi sarcoma was significantly higher than that in plaque stage (P0.05). Conclusion: the detection of human herpesvirus 8 by immunohistochemical method is of great significance in the diagnosis of Kaposi sarcoma, especially in differential diagnosis.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R739.5

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