磷脂酰肌醇3激酶调节亚基2在卡波西肉瘤中的表达及意义

发布时间：2018-08-07 15:02

【摘要】：目的:明确卡波西肉瘤中磷脂酸肌醇3调节亚基2(PIK3R2)蛋白及mRNA的表达水平,并分析PIK3R2表达与卡波西肉瘤不同临床分期及病理特征的关系。方法:选取维吾尔族卡波西肉瘤患者30例和健康者24例的皮损石蜡包埋组织,采用免疫组织化学和实时荧光定量聚合酶链式反应(qPCR)方法检测PIK3R2的表达水平,分析与性别、年龄、病理分期、皮损面积、HIV感染等临床病理特征间关系。结果:卡波西肉瘤组中PIK3R2的蛋白表达阳性率为60%,正常皮肤组中为25%,有统计学意义(P0.05)。在卡波西肉瘤组内斑片期阳性表达率33.3%,斑块期阳性率71.4%,结节期阳性率81.8%,有统计学意义(P0.05),后两组间无统计学差异(P0.05),其余两两比较均有统计学差异,病理分期与蛋白表达量呈正相关(P0.05),在性别、年龄、皮损面积、HIV及HHV-8感染情况等方面无差异(P0.05)。卡波西肉瘤组中PIK3R2的mRNA表达水平(2.92±0.56)显著高于正常皮肤组(1.13±0.82),差异有统计学意义(P0.05),PIK3R2 mRNA的表达量在卡波西肉瘤组织斑片期、斑块期、结节期分别为2.37±0.20,2.83±0.54,3.56±0.23,三组之间差异均有统计学意义(P0.05),病理分期与m RNA表达量呈正相关(P0.05),但与卡波西肉瘤患者性别、年龄、皮损面积、HIV及HHV-8感染情况无关(P0.05)。结论:PIK3R2在卡波西肉瘤组织中的呈现高表达,PIK3R2的蛋白及m RNA表达水平在斑片期、斑块期、结节期呈现递增趋,呈正相关性,PIK3R2表达水平与患者的性别、年龄、皮损面积、HIV及HHV-8感染情况无关,PIK3R2可为寻找卡波西肉瘤的分子生物诊断标记提供一定基础,为判断卡波西肉瘤的病理演变提供新思路、新线索。
[Abstract]:Objective: to determine the expression of phosphatidylinositol 3 mediated subunit 2 (PIK3R2) protein and mRNA in the sarcoma of card Posey's sarcoma, and to analyze the relationship between PIK3R2 expression and the different clinical stages and pathological features of Posey sarcoma. Methods: 30 cases of Uygur card Posey sarcoma and 24 cases of healthy persons were selected to be embedded in paraffin embedded tissue, and immunohistochemistry was used. The expression level of PIK3R2 was detected by real time fluorescence quantitative polymerase chain reaction (qPCR), and the relationship between the sex, age, pathological stage, skin lesion area, HIV infection and other clinicopathological features was analyzed. Results: the positive rate of PIK3R2 protein expression in the group of card Posey's sarcoma was 60% and 25% in the normal skin group (P0.05). In card Posey The positive rate of patch stage in the sarcoma group was 33.3%, the positive rate of plaque stage was 71.4%, the positive rate of nodular stage was 81.8%, with statistical significance (P0.05). There was no statistical difference between the two groups (P0.05), the other 22 were statistically different, the pathological stage and the protein expression Cheng Zhengxiang (P0.05), in sex, age, skin lesion area, HIV and HHV-8 infection There was no difference in the other aspects (P0.05). The level of mRNA expression of PIK3R2 in the carPosey's sarcoma group was significantly higher than that of the normal skin group (1.13 + 0.82), the difference was statistically significant (P0.05). The expression of PIK3R2 mRNA was 2.37 + 0.20,2.83 + 0.54,3.56 + 0.23, respectively, in the tissue plaque stage of the sarcoma of the card Posey's sarcoma, respectively, and the three groups were different among the three groups. Statistical significance (P0.05), the pathological staging was positively correlated with the expression of M RNA (P0.05), but was not related to the sex, age, skin lesion area, HIV and HHV-8 infection in patients with card Posey's sarcoma (P0.05). Conclusion: PIK3R2 is highly expressed in the sarcoma of the card, and the expression level of PIK3R2 protein and m RNA is at the stage of patch, patch period, and nodular phase. There is a positive correlation. The level of PIK3R2 expression is not related to the sex, age, skin lesion area, HIV and HHV-8 infection. PIK3R2 can provide a certain basis for the molecular biomarkers for finding the card Posey's sarcoma, and provide new clues for judging the pathological changes of Capocci's sarcoma.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.5

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