转录因子RORγt和Foxp3在玫瑰糠疹患者外周血单个核细胞中的表达

发布时间：2018-08-13 09:25

【摘要】：目的:了解Th17细胞、Treg细胞特异性转录因子RORγt(RORγt mRNA)、Foxp3(Foxp3 mRNA)在急性期玫瑰糠疹(PR)患者外周血单个核细胞中的表达水平,探讨其在玫瑰糠疹急性期发病中的作用。方法:采用荧光实时定量反转录聚合酶链反应(RT-PCR)检测20例玫瑰糠疹急性期患者(PR组)和20例健康体检者(健康对照组)外周血单个核细胞中RORγt和Foxp3mRNA的表达水平。结果:PR组RORγt mRNA、Foxp3 mRNA的表达水平均显著高于健康对照组,差异有统计学意义(t值分别是3.140、2.516,P均0.05);PR组RORγt/Foxp3 mRNA表达水平的比值(2.31±0.90)增大,与健康对照组(0.86±0.40)比较,差异有统计学意义(t=4.445,P0.01)。PR组RORγt mRNA与Foxp3 mRNA的表达水平呈正相关(r=0.811,P0.01);健康对照组RORγt mRNA与Foxp3 mRNA表达水平无相关性(r=-0.253,P0.05)。RORγt mRNA表达水平与玫瑰糠疹严重程度评分(18.33±7.56)呈正相关(r=0.634,P0.05),Foxp3 mRNA表达水平与玫瑰糠疹严重程度评分(18.33±7.56)无相关性(r=0.171,P0.05)。结论:玫瑰糠疹患者外周血RORγt mRNA与Foxp3 mRNA的表达水平均显著增高,且以RORγt mRNA的表达占优。Th17/Treg细胞比例失衡,可能在玫瑰糠疹的发病中起着一定的作用。
[Abstract]:Objective: to investigate the expression of ROR 纬 t (ROR 纬 t mRNA) Foxp3 (Foxp3 mRNA) in peripheral blood mononuclear cells (PBMC) of patients with pityriasis roseus (PR) in order to explore the role of Foxp3 mRNA in the pathogenesis of pityriasis roseus. Methods: the expression of ROR 纬 t and Foxp3mRNA in peripheral blood mononuclear cells (PBMC) of 20 patients with pityriasis roseus (PR group) and 20 healthy controls (healthy control group) was detected by fluorescence real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR). Results the expression level of ROR 纬 t mRNA-Foxp3 mRNA in the PR group was significantly higher than that in the healthy control group (t = 3.140 卤2.516, respectively). The ratio of ROR 纬 t/Foxp3 mRNA expression in the PR group (2.31 卤0.90) was significantly higher than that in the healthy control group (0.86 卤0.40). The difference was statistically significant (tr 4.445, P0.01). There was a positive correlation between the expression of ROR 纬 t mRNA and Foxp3 mRNA in PR group (r = 0.811, P 0.01), and no correlation between ROR 纬 t mRNA and Foxp3 mRNA expression in healthy control group (r = 0.253 P 0.05) .ROR 纬 t mRNA expression level was positively correlated with the severity score of pityriasis roseus (18.33 卤7.56) (r 0.634 P 0.05). There was no correlation between flat and severity score of pityriasis roseus (18.33 卤7.56) (r = 0.171 P 0.05). Conclusion: the levels of ROR 纬 t mRNA and Foxp3 mRNA in peripheral blood of patients with pityriasis roseus were significantly increased, and the imbalance of the proportion of Th17% Treg cells in the expression of ROR 纬 t mRNA may play a role in the pathogenesis of pityriasis roseus.
【作者单位】：郑州大学第二附属医院皮肤科;
【基金】：河南省教育厅基金(14A320055)资助项目
【分类号】：R758.64

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