纳米氧化锌对小鼠B16黑素瘤细胞生长和分化的影响

发布时间：2018-08-14 15:34

【摘要】：为评价纳米氧化锌(Zn O NPs)在皮肤病治疗和化妆品应用方面的生理毒性,以小鼠B16黑素瘤细胞为细胞模型,研究15、30、90 nm下Zn O NPs对B16细胞的体外生长和分化的影响及其作用机制。以不加纳米氧化锌和加体相氧化锌的处理分别作阴性、阳性对照。结果表明,15、30 nm,体相Zn O NPs处理与B16细胞共培养作用3 d,当Zn O NPs为4μg/m L以下时,Zn O NPs并未明显抑制细胞生长,引起细胞死亡;当Zn O NPs浓度大于8μg/m L时,明显抑制细胞生长。随着Zn O NPs浓度增加,细胞体积变小,细胞间隙变大,黏附性降低,细胞变圆,从培养瓶底脱落死亡。Zn O NPs诱导细胞中酪氨酸酶(TYR)、酪氨酸酶相关蛋白1(TYRP-1)、酪氨酸相关酶相关蛋白2(TYRP-2)的mRNA表达量升高,导致细胞内酪氨酸酶活性和黑色素生成水平增高,促进了B16黑素瘤细胞的分化。相同Zn O NPs浓度下,15 nm Zn O NPs比30、90 nm Zn O NPs作用明显。
[Abstract]:In order to evaluate the physiological toxicity of nano-zinc oxide (Zn O NPs) in the treatment of skin diseases and cosmetic application, the effect of Zn O NPs on the growth and differentiation of B16 cells at 153090 nm and its mechanism were studied using mouse B16 melanoma cells as cell model. The treatment of no nanometer zinc oxide and body phase zinc oxide were used as negative and positive control respectively. The results showed that the growth of B16 cells was inhibited by Zno NPs when Zn O NPs was less than 4 渭 g / mL for 3 days, and when Zn O NPs concentration was more than 8 渭 g / mL, the growth of B16 cells was inhibited obviously when Zn O NPs concentration was more than 8 渭 g / mL. With the increase of Zn O NPs concentration, the cell volume becomes smaller, the cell gap becomes larger, the adhesion decreases, and the cell becomes round. The expression of tyrosinase (TYR), tyrosinase associated protein 1 (TYRP-1) and tyrosinase associated protein 2 (TYRP-2) mRNA was increased in culture bottle bottom death. Zn O NPs induced the increase of tyrosinase activity and melanin production. It promoted the differentiation of B 16 melanoma cells. At the same concentration of Zn O NPs, the effect of 15 nm Zn O NPs was more obvious than that of 30 nm Zn O NPs.
【作者单位】：苏州大学基础医学与生物科学学院;
【基金】：国家自然科学基金(编号:81071306)
【分类号】：R739.5

【共引文献】