两种人工合成抗原在麻风血清学诊断中的再评价

发布时间：2018-08-15 16:33

【摘要】：目的评价两种人工抗原ND-O-BSA(ND)和NT-P-BSA(NT)在麻风血清学诊断中的价值。方法分别用ND-ELISA和NT-ELISA检测麻风患者、正常人及其他个体血清中的IgM和IgG,绘制ROC曲线(receiver operating characteristic curve)并计算诊断麻风的最佳临界值。用散点图显示OD值与细菌指数(BI)之间的关系。用ND-IgM-ELISA和NT-IgM-ELISA连续2年监测50名已治愈麻风患者血清中的抗体水平。结果 ND-IgM-ELISA,NT-IgM-ELISA,ND-IgG-ELISA和NT-IgG-ELISA 4组实验的ROC曲线下面积分别为0.98,0.95,0.88和0.82;cutoff值分别为0.14,0.20,0.19和0.24;敏感性分别为96.20%,85.00%,82.80%和80.00%;特异性分别为97.50%,95.00%,83.75%和76.25%。OD值与BI呈正相关。50名已治愈患者连续2年的监测中发现有6名血清抗体水平高于临界值。结论 NDIgM-ELISA实验的敏感性和特异性是最好的,且不存在交叉反应,其灵敏度高于查菌方法,可以早期检测到麻风抗体。其他三种方法仍可用于麻风血清抗体水平的监测。
[Abstract]:Objective to evaluate the value of two artificial antigens (ND-O-BSA (ND) and NT-P-BSA (NT) in the diagnosis of leprosy. Methods ND-ELISA and NT-ELISA were used to detect IgM and IgG in the serum of leprosy patients, normal persons and other individuals respectively. The (receiver operating characteristic curve) curve of ROC was plotted and the best critical value for the diagnosis of leprosy was calculated. The relationship between OD value and bacterial index (BI) was shown by scatter plot. Serum antibody levels in 50 cured leprosy patients were monitored by ND-IgM-ELISA and NT-IgM-ELISA for 2 consecutive years. Results the area under the ROC curve of ND-IgM-ELISA-ND-IgG-ELISA and ND-IgG-ELISA and NT-IgG-ELISA 4 groups were 0.140.200.200.19 and 0.24.The sensitivity was 96.200.200.80% and 80.004.The specificity was 97.50,95.000.0083.75% and the 76.25%.OD value was positively correlated with BI. 50 cured patients were monitored for two consecutive years. Serum antibody levels were found to be above the threshold in 6 patients. Conclusion the sensitivity and specificity of NDIgM-ELISA assay are the best, and there is no cross reaction. The sensitivity of NDIgM-ELISA assay is higher than that of bacteriological test, and leprosy antibody can be detected early. The other three methods can still be used to monitor the serum antibody level of leprosy.
【作者单位】：大连市皮肤病医院;
【分类号】：R755

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