2%酮康唑乳膏、2%硝酸舍他康唑乳膏、1%联苯苄唑乳膏的体外抗真菌活性研究

发布时间：2018-08-20 10:39

【摘要】：目的：通过对2%酮康唑乳膏、2%硝酸舍他康唑乳膏、1%联苯苄唑乳膏的体外药敏实验分析3种咪唑类抗真菌药物的体外抗真菌活性。从而为这3种药物的临床使用提供依据，指导临床用药。方法：选用临床分离致病真菌共5种50株，用琼脂扩散法进行体外药敏实验。制备含2%琼脂的马铃薯琼脂含菌培养基，将3种药物等体积的打入含菌平板的加药孔。28℃恒温培养10天后测量每种药周围的抑菌环半径，综合数据进行统计学分析。结果：2%酮康唑乳膏、2%硝酸舍他康唑乳膏、1%联苯苄唑乳膏对3种皮肤癣菌菌株40株、2种念珠菌菌株10株共5种50株菌株均形成抑菌圈。2%酮康唑乳膏的抑菌圈半径均数为13.72mm，2%硝酸舍他康唑乳膏的抑菌圈半径均数为12.35mm、1%联苯苄唑乳膏的抑菌圈半径均数为6.94mm。结论：通过对2%酮康唑乳膏、2%硝酸舍他康唑乳膏、1%联苯苄唑乳膏的体外药敏实验，得出对于所有实验菌株，3种抗真菌药物的抗真菌活性均有统计学差异，抗真菌活性由强到弱依次为：2%酮康唑乳膏2%硝酸舍他康唑乳膏1%联苯苄唑乳膏。对于皮肤癣菌3种药的抗真菌活性为：2%酮康唑乳膏2%硝酸舍他康唑乳膏1%联苯苄唑乳膏；对于念珠菌3种药的抗真菌活性为：2%酮康唑乳膏与2%舍他康唑乳膏差异无统计学意义，，但二者均优于1%联苯苄唑乳膏。
[Abstract]:Aim: to analyze the in vitro antifungal activity of three imidazole antifungal agents by in vitro drug sensitivity test of 2% ketoconazole cream and 2% seraconazole nitrate cream and 1% bifonazole cream. So as to provide the basis for the clinical use of these three drugs, guiding clinical use. Methods: 50 strains of 5 kinds of pathogenic fungi were isolated and tested by Agar diffusion in vitro. Potato Agar culture medium containing 2% Agar was prepared. After 10 days of constant temperature culture, the radius of bacteriostasis ring around each drug was measured, and the comprehensive data were analyzed statistically. Results 2% 2% ketoconazole cream and 2% sertaconazole nitrate 1% bifonazole cream formed inhibition circle radius of 3 strains of dermatophytes 40 strains, 2 strains of Candida, 10 strains of 5 species of 50 strains. 2% ketoconazole cream. 2% ketoconazole cream, 2% ketoconazole cream. The mean radius of bacteriostasis circle was 12.35 mm ~ (-1) bifonazole cream with a mean of 13.72 mm ~ (-2) nitrateconazole nitrate and 6.94 mm ~ (-1) bifonazole cream. Conclusion: the in vitro drug sensitivity test of 2% ketoconazole cream and 2% seraconazole nitrate cream and 1% bifonazole cream showed that there were significant differences in the antifungal activities among the three antifungal drugs against all experimental strains. The order of antifungal activity from strong to weak is: 1% bifonazole cream 2% ketoconazole cream 2% sertaconazole nitrate cream 1% bifonazole cream. The antifungal activity of these three drugs was 1% bifonazole cream, 2% ketoconazole cream, 2% saltaconazole nitrate cream, 1% bifonazole cream, 2% ketoconazole cream, 2% oxaconazole cream, 1% bifonazole cream. There was no significant difference in the antifungal activity of 2% ketoconazole cream and 2% sertaconazole cream against Candida, but both of them were superior to 1% bifonazole cream.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R756

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