基于双光子激发荧光与二次谐波相结合的非线性光谱成像技术对小儿毛细血管瘤的研究

发布时间：2018-08-22 10:08

【摘要】：目的:探讨基于双光子激发荧光(TPEF)与二次谐波信号(SHG)相结合的非线性光谱成像方法对在临床上用于无损探测、实时监测小儿毛细血管瘤的病理生理过程,追踪其病程发展过程,指导治疗方法以及评估治疗效果的潜力。方法:选取58例我科2009年7月到2010年4月以来住院患儿皮肤毛细血管瘤(颌面部)手术切除标本,经病理证实:其中增生期(2-12个月)26例,消退期(2-5岁)16例,消退完成期(6-11岁)16例。将样品切成30um厚度,进行非线性光谱成像分析,并将同一患者的正常皮肤做为对照。利用非线性光谱成像系统对样品进行成像及光谱分析。结果:正常皮肤与增生期毛细血管瘤组织中的胶原蛋白的含量、密度以及弹性蛋白纤维的形态结构、分布、排列规律特点上存在很大差异。消退期时差异明显减小,消退完成期时与正常皮肤无显著差异。增生期、消退期、消退完成期毛细血管瘤组织中胶原蛋白SHG信号强度与相应的正常皮肤相比逐渐减小。增生期、消退期、消退完成期毛细血管瘤组织中胶原蛋白深度依赖衰减常数与正常皮肤相比,差别逐渐减少。病变组织中弹性纤维直径逐渐增大,直到与正常皮肤无差别。结论:所有的测量数据及获得的非线性光谱图像表明,正常皮肤与不同时期毛细血管瘤组织之间存在的差异,而这些差异可作为区分正常皮肤与不同时期毛细血管瘤组织的重要指标,基于双光子激发荧光和二次谐波信号相结合的非线性光谱成像技术可能成为临床上用于无损探测、实时监测小儿毛细血管瘤的病理生理过程,追踪其病程发展过程,指导治疗方案以及评估治疗效果的有力工具。
[Abstract]:Objective: to investigate the clinical application of nonlinear spectral imaging based on two-photon excited fluorescence (TPEF) (TPEF) combined with second-harmonic signal (SHG) to detect the pathophysiological process of pediatric capillary hemangioma in real time and to track the course of the disease. Guide treatment methods and evaluate the potential of treatment effectiveness. Methods: 58 surgical specimens of skin capillary hemangioma (maxillofacial region) from July 2009 to April 2010 in our department were selected. According to pathology, 26 cases were in proliferative stage (2-12 months), 16 cases were in regression phase (2-5 years old). There were 16 cases of complete extinction (6-11 years old). The samples were cut into 30um thickness and analyzed by nonlinear spectral imaging, and the normal skin of the same patient was used as a control. The nonlinear spectral imaging system was used to image and analyze the samples. Results: there were significant differences between normal skin and proliferative capillary hemangioma in the content of collagen, density and the morphological structure, distribution and arrangement of elastin fibers. There was no significant difference between the complete stage of regression and normal skin. The signal intensity of collagen SHG in capillary hemangioma at proliferative and complete stage decreased gradually compared with the corresponding normal skin. The depth dependent attenuation constant of collagen in capillary hemangioma in proliferative and complete phase decreased gradually compared with that in normal skin. The diameter of the elastic fibers in the lesions gradually increased until they were no different from the normal skin. Conclusion: all the measured data and the obtained nonlinear spectral images show that there are differences between normal skin and capillary hemangioma at different stages. These differences can be used as an important index to distinguish normal skin from capillary hemangioma at different stages. Non-linear spectral imaging based on two-photon excitation fluorescence and second harmonic signal may be used in clinical nondestructive detection. To monitor the pathophysiological process of capillary hemangioma in children in real time, to track the course of the disease, to guide the treatment plan and to evaluate the therapeutic effect.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.5

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