牵张力作用下瘢痕疙瘩间充质干细胞对成纤维细胞增殖及胶原合成的影响
发布时间：2018-09-07 09:21
【摘要】：目的研究牵张力作用下瘢痕疙瘩间充质干细胞(keloid-derived mesenchymal stem cells,KD-MSCs)培养上清对瘢痕疙瘩成纤维细胞(keloid fibroblasts,KFs)增殖和胶原合成的影响,探讨牵张力及KD-MSCs在瘢痕疙瘩形成过程中的作用。方法培养原代KD-MSCs和KFs,于72h收集牵张力作用下和未施加牵张力的KD-MSCs培养上清,并分别孵育KFs 24h,48h,72h,96h,倒置相差显微镜观察细胞的形态学变化,CCK-8法检测KFs的增殖活力,Real-time PCR检测KFs中Ⅰ型和Ⅲ型胶原和CTGF mRNA转录情况,羟脯氨酸消化法检测胶原水平的变化。结果培养上清刺激后,两组间KFs形态未见明显差异;CCK-8结果提示牵张力组KFs增殖明显强于无牵张力组(P0.05);Real-time PCR结果示牵张力组KFsⅠ型胶原的mRNA表达明显高于无牵张力组(P0.01),牵张力组KFsⅢ型胶原的mRNA表达低于无牵张力组(P0.05),牵张力组KFs CTGF的mRNA表达明显低于无牵张力组(P0.01);羟脯氨酸检测提示牵张力组KFs羟脯氨酸含量明显高于无牵张力组(P0.05)。结论牵张力作用可明显增强KD-MSCs培养上清对KFs增殖和胶原合成的促进作用,提示牵张力作用下KD-MSCs可发生旁分泌促进成纤维细胞增生和分泌,从而参与瘢痕疙瘩的形成,其具体机制仍需深入研究。
[Abstract]:Objective to study the effects of the culture supernatant of keloid mesenchymal stem cells (keloid-derived mesenchymal stem cells,KD-MSCs) on the proliferation and collagen synthesis of keloid fibroblasts (keloid fibroblasts,KFs) and the role of KD-MSCs in the process of keloid formation. Methods the supernatants of primary KD-MSCs and KFs, were collected under and without stretch for 72 h. The morphological changes of cells were observed by inverted phase contrast microscope. The proliferative activity of KFs was detected by CCK-8 method Real-time PCR was used to detect the transcription of type 鈪，
本文编号：2227844
[Abstract]:Objective to study the effects of the culture supernatant of keloid mesenchymal stem cells (keloid-derived mesenchymal stem cells,KD-MSCs) on the proliferation and collagen synthesis of keloid fibroblasts (keloid fibroblasts,KFs) and the role of KD-MSCs in the process of keloid formation. Methods the supernatants of primary KD-MSCs and KFs, were collected under and without stretch for 72 h. The morphological changes of cells were observed by inverted phase contrast microscope. The proliferative activity of KFs was detected by CCK-8 method Real-time PCR was used to detect the transcription of type 鈪，
本文编号：2227844
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