GCDFP-15及p63在Paget病和Paget样Bowen病中的表达及意义
发布时间：2018-09-09 20:38
【摘要】：目的:观察GCDFP-15与p63在Paget病与Paget样Bowen病中的表达情况及意义。 方法:选择资料完整的1996年~2010年吉林大学第二医院皮肤科病理诊断的Paget病和Paget样Bowen病,其中乳房Paget病10例,均为女性,乳外Paget病15例,14例为男性,1例女性。Paget病GCDFP-15阳性片及普通Bowen病的表皮p63阳性片作为各标志的阳性对照;PBS液代替一抗的Paget病和Paget样Bowen病作为阴性对照,对两种疾病进行HE染色和p63、GCDFP-15的免疫组化(SP法)染色,DAB显色。结果判定:GCDFP-15为胞浆着色,p63胞核着色,Paget病中Paget细胞被染为棕褐色即判为阳性;Paget样Bowen病中Paget样细胞被染成棕褐色即判定阳性。 结果:10例MPD中3例GCDFP-15阳性表达(3/10),15例EMPD中4例GCDFP-15阳性表达(4/15),25例Paget病中共7例阳性表达;11例Paget样Bowen病中GCDFP-15全部阴性。P63在25例Paget病中全部阴性,11例Paget样Bowen病10例阳性。 结论:1、Paget病中GCDFP-15阳性率较低,阳性时可以鉴别Paget病与Paget样Bowen病,阴性时则不能鉴别,敏感性较低。2、p63在Bowen病中过表达,空泡细胞的阳性率较高,Paget病中Paget细胞阴性表达或阳性率较低,p63可以用来鉴别Paget病和Paget样Bowen病,且认为优于GCDFP-15。
[Abstract]:Objective: to observe the expression and significance of GCDFP-15 and p63 in Paget disease and Paget like Bowen disease. Methods: Paget disease and Paget like Bowen disease diagnosed by pathology in Department of Dermatology, second Hospital of Jilin University from 1996 to 2010 were selected. 10 cases of Paget disease of breast were all female. Fifteen patients with extramammary Paget's disease (14 / 14) were male / female with GCDFP-15 positive film and common Bowen's disease with epidermis p63 positive film instead of the first antibody Paget's disease and Paget's like Bowen's disease as the negative control, and the epidermis p63 positive film of the common Bowen's disease was used as the negative control. The two diseases were stained with HE and p63 GCDFP-15 by SP staining. Results Paget cells were found to be positive in Paget's disease in which Paget cells were stained brown. Paget like cells were found to be positive in Paget's disease. Results in 10 cases of MPD, 3 cases (3 / 10) of GCDFP-15 positive expression (3 / 10) and 15 cases of EMPD, 4 cases (4 / 15) of Paget disease, 7 cases of Paget like Bowen disease, 7 cases of GCDFP-15 negative. P63 were all negative in 25 cases of Paget disease and 11 cases of Paget like Bowen disease. Conclusion the positive rate of GCDFP-15 is lower in the disease of Paget. It can differentiate Paget disease from Paget like Bowen disease when it is positive, but it can not be distinguished when it is negative, and the sensitivity is low. 2p63 is overexpressed in Bowen disease. The positive rate of vacuolar cells is higher. The negative expression or positive rate of Paget cells in Paget's disease is lower. P63 can be used to differentiate Paget disease from Paget like Bowen disease, and it is better than GCDFP-15..
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.5
本文编号：2233505
[Abstract]:Objective: to observe the expression and significance of GCDFP-15 and p63 in Paget disease and Paget like Bowen disease. Methods: Paget disease and Paget like Bowen disease diagnosed by pathology in Department of Dermatology, second Hospital of Jilin University from 1996 to 2010 were selected. 10 cases of Paget disease of breast were all female. Fifteen patients with extramammary Paget's disease (14 / 14) were male / female with GCDFP-15 positive film and common Bowen's disease with epidermis p63 positive film instead of the first antibody Paget's disease and Paget's like Bowen's disease as the negative control, and the epidermis p63 positive film of the common Bowen's disease was used as the negative control. The two diseases were stained with HE and p63 GCDFP-15 by SP staining. Results Paget cells were found to be positive in Paget's disease in which Paget cells were stained brown. Paget like cells were found to be positive in Paget's disease. Results in 10 cases of MPD, 3 cases (3 / 10) of GCDFP-15 positive expression (3 / 10) and 15 cases of EMPD, 4 cases (4 / 15) of Paget disease, 7 cases of Paget like Bowen disease, 7 cases of GCDFP-15 negative. P63 were all negative in 25 cases of Paget disease and 11 cases of Paget like Bowen disease. Conclusion the positive rate of GCDFP-15 is lower in the disease of Paget. It can differentiate Paget disease from Paget like Bowen disease when it is positive, but it can not be distinguished when it is negative, and the sensitivity is low. 2p63 is overexpressed in Bowen disease. The positive rate of vacuolar cells is higher. The negative expression or positive rate of Paget cells in Paget's disease is lower. P63 can be used to differentiate Paget disease from Paget like Bowen disease, and it is better than GCDFP-15..
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.5
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