Ezrin和AQP3在皮肤鳞状细胞癌中的表达
发布时间：2018-10-08 06:16
【摘要】：目的： 皮肤鳞状细胞癌(squamous cell carcinoma, SCC)是起源于角质形成细胞或附属器鳞状上皮细胞的恶性肿瘤,是最常见的皮肤恶性肿瘤之一,具有侵袭性,易于发生临近或远位转移,尤其转移到重要的器官,从而危及生命。该病的发生、侵袭和转移机制目前仍不十分清楚,因此研究皮肤鳞状细胞癌侵袭和转移的机理,对于肿瘤的预防和治疗具有重要意义。 埃兹蛋白(Ezrin protein, Ezrin)是将细胞膜蛋白与肌动蛋白-细胞骨架连接起来的桥接蛋白,具有维持细胞形态和运动、连接黏附分子及调节信号转导等功能。目前研究发现,其主要通过与肿瘤细胞表面黏附分子发生作用来影响肿瘤的分化、侵袭性和转移；水通道蛋白(aquaporins, AQPs)是一类能快速转运水的膜整合蛋白,皮肤上主要分布的水通道蛋白是水通道蛋白3 (aquaporin-3,AQP3)。目前发现AQP3参与多种生理病理过程,其高表达与恶性肿瘤的侵袭和转移有着密切的联系。 检测Ezrin蛋白,AQP3在皮肤鳞状细胞癌中的表达,探讨它们在皮肤鳞状细胞癌发生、发展中的意义及其与临床病理特征的相关性,并探讨二者的相互关系以及在皮肤鳞状细胞癌发病中所起的作用,以期为皮肤鳞状细胞癌的发病机制提供理论依据。 材料与方法： 收集石蜡包埋的皮肤鳞状细胞癌标本43例,其中高分化16例,中分化15例,低分化12例。对照组为15例正常皮肤。应用免疫组化SP法检测43例皮肤鳞状细胞癌和15例正常皮肤组织中Ezrin蛋白和AQP3的表达水平,用已知的Ezrin和AQP3阳性的皮肤鳞状细胞癌标本做为阳性对照,以PBS代替一抗作为阴性对照；结合临床病理学资料应用SPSS13.0统计软件包进行统计学分析。 结果： Ezrin蛋白在43例皮肤鳞状细胞癌中阳性率为93%,15例正常皮肤组织中Ezrin蛋白阳性表达率为6.67%,两者比较差异有显著性(P0.01);Ezrin蛋白的表达与肿瘤的分化程度有显著相关性,其阳性率随皮肤鳞状细胞癌病理分级的降低而升高,差异有显著性(P0.01)；Ezrin蛋白在皮肤鳞状细胞癌中则以细胞质染色或细胞膜、细胞质均着色为主。AQP3在皮肤鳞状细胞癌和正常皮肤中的阳性表达率分别为88.4%和6.67%,差异有显著性(P0.01)；在皮肤鳞状细胞癌中AQP3蛋白的表达与肿瘤的分化程度有显著相关性(P0.01),即随皮肤鳞状细胞癌病理分级的增高而升高；AQP3在皮肤鳞状细胞癌中则以细胞质染色或细胞膜、细胞质均着色为主。Ezrin蛋白和AQP3的表达呈显著正相关,有统计学意义(r=0.573,P0.01)。 结论： 1、Ezrin蛋白在皮肤鳞状细胞癌中表达增高,且表达水平在皮肤鳞状细胞癌中与肿瘤的分化程度呈负相关,即分化越差,表达越高。 2、AQP3在皮肤鳞状细胞癌中表达增高,且表达水平在皮肤鳞状细胞癌中与肿瘤的分化程度呈正相关,即分化越高,表达越高。 3、Ezrin蛋白和AQP3在皮肤鳞状细胞癌中的表达呈正相关,表明二者在皮肤鳞状细胞癌的发生、发展过程中可能具有协同作用。
[Abstract]:Purpose: Skin squamous cell carcinoma (SCC) is a malignant tumor derived from keratinocyte or accessory squamous epithelial cells. It is one of the most common malignant tumors. It is invasive, prone to imminent or distant metastasis, and is especially important. Organ, thereby endangering The pathogenesis, invasion and metastasis mechanism of the disease is still not very clear, so it is important to study the mechanism of invasion and metastasis of squamous cell carcinoma of the skin, and it is important for the prevention and treatment of tumor. Significance. Ezrin (Ezrin) is a bridging protein that connects a cell membrane protein with actin-actin, which has the ability to maintain cell morphology and movement, attach adhesion molecules, and modulate signals. transduction, etc. It has been found that it mainly affects the differentiation, invasion and metastasis of tumor by adhesion molecule on the surface of tumor cells, and aquaporin (AQPs) is a kind of rapid transit water. the membrane whole protein, the main distribution of water channel protein on the skin is aquaporin-3, AQP3 (AQP3). It is found that AQP3 is involved in many physiological and pathological processes, and its high expression is related to the invasion and metastasis of malignant tumor. To investigate the expression of Ezrin protein and AQP3 in the squamous cell carcinoma of the skin, to explore their significance in the development of squamous cell carcinoma of the skin and their correlation with clinicopathological features, and to explore the relationship between them and the squamous cell carcinoma of the skin. The role of the disease in the pathogenesis of squamous cell carcinoma of the skin. mechanism-providing theory Materials and Methods: 43 cases of paraffin-embedded squamous cell carcinoma of the skin were collected, in which 16 cases were highly differentiated. Medium differentiation: 15 cases, low differentiation 1 The expression levels of Ezrin protein and AQP3 in 43 cases of skin squamous cell carcinoma and 15 normal skin tissues were detected by immunohistochemical SP method. Use PBS instead of an anti-negative control; use SPSS13 in combination with clinical pathology data. 0 Series Results: The positive rate of Ezrin protein in 43 cases of skin squamous cell carcinoma was 93%, the positive rate of Ezrin protein in 15 normal skin tissues was 6.67%, and the difference was significant (P0.01); Ezri There was a significant correlation between the expression of n-protein and the degree of differentiation of tumor. The positive rate of n-protein increased with the decrease of pathological grade of squamous cell carcinoma of the skin (P <0.01); Ezrin protein was found in squamous cell carcinoma of the skin The positive expression rates of AQP3 in skin squamous cell carcinoma and normal skin were 88. 4% and 6.67%, respectively (P <0.01). The degree of differentiation of tumor was significantly correlated (P0.01), that is, increased with the increase of pathological grade of squamous cell carcinoma of the skin; AQP3 was found in squamous cell carcinoma of the skin The expression of Ezrin protein and AQP3 was positively correlated with the expression of Ezrin protein and AQP3. Statistics Conclusion: 1. The expression of Ezrin protein in squamous cell carcinoma of the skin is increased, and the expression level is in the skin squamous cell carcinoma. There is a negative correlation with the degree of differentiation of the tumor, that is, the poorer the differentiation, the higher the expression. The expression of AQP3 in the squamous cell carcinoma of the skin is increased, and the expression level is at the skin The expression of Ezrin protein and AQP3 in squamous cell carcinoma of the skin is positive.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.5
[Abstract]:Purpose: Skin squamous cell carcinoma (SCC) is a malignant tumor derived from keratinocyte or accessory squamous epithelial cells. It is one of the most common malignant tumors. It is invasive, prone to imminent or distant metastasis, and is especially important. Organ, thereby endangering The pathogenesis, invasion and metastasis mechanism of the disease is still not very clear, so it is important to study the mechanism of invasion and metastasis of squamous cell carcinoma of the skin, and it is important for the prevention and treatment of tumor. Significance. Ezrin (Ezrin) is a bridging protein that connects a cell membrane protein with actin-actin, which has the ability to maintain cell morphology and movement, attach adhesion molecules, and modulate signals. transduction, etc. It has been found that it mainly affects the differentiation, invasion and metastasis of tumor by adhesion molecule on the surface of tumor cells, and aquaporin (AQPs) is a kind of rapid transit water. the membrane whole protein, the main distribution of water channel protein on the skin is aquaporin-3, AQP3 (AQP3). It is found that AQP3 is involved in many physiological and pathological processes, and its high expression is related to the invasion and metastasis of malignant tumor. To investigate the expression of Ezrin protein and AQP3 in the squamous cell carcinoma of the skin, to explore their significance in the development of squamous cell carcinoma of the skin and their correlation with clinicopathological features, and to explore the relationship between them and the squamous cell carcinoma of the skin. The role of the disease in the pathogenesis of squamous cell carcinoma of the skin. mechanism-providing theory Materials and Methods: 43 cases of paraffin-embedded squamous cell carcinoma of the skin were collected, in which 16 cases were highly differentiated. Medium differentiation: 15 cases, low differentiation 1 The expression levels of Ezrin protein and AQP3 in 43 cases of skin squamous cell carcinoma and 15 normal skin tissues were detected by immunohistochemical SP method. Use PBS instead of an anti-negative control; use SPSS13 in combination with clinical pathology data. 0 Series Results: The positive rate of Ezrin protein in 43 cases of skin squamous cell carcinoma was 93%, the positive rate of Ezrin protein in 15 normal skin tissues was 6.67%, and the difference was significant (P0.01); Ezri There was a significant correlation between the expression of n-protein and the degree of differentiation of tumor. The positive rate of n-protein increased with the decrease of pathological grade of squamous cell carcinoma of the skin (P <0.01); Ezrin protein was found in squamous cell carcinoma of the skin The positive expression rates of AQP3 in skin squamous cell carcinoma and normal skin were 88. 4% and 6.67%, respectively (P <0.01). The degree of differentiation of tumor was significantly correlated (P0.01), that is, increased with the increase of pathological grade of squamous cell carcinoma of the skin; AQP3 was found in squamous cell carcinoma of the skin The expression of Ezrin protein and AQP3 was positively correlated with the expression of Ezrin protein and AQP3. Statistics Conclusion: 1. The expression of Ezrin protein in squamous cell carcinoma of the skin is increased, and the expression level is in the skin squamous cell carcinoma. There is a negative correlation with the degree of differentiation of the tumor, that is, the poorer the differentiation, the higher the expression. The expression of AQP3 in the squamous cell carcinoma of the skin is increased, and the expression level is at the skin The expression of Ezrin protein and AQP3 in squamous cell carcinoma of the skin is positive.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.5
【相似文献】
相关期刊论文 前10条
1 马颖;杨向红;高霭峰;林连捷;;大肠肿瘤中Ezrin的表达及意义[J];现代肿瘤医学;2011年07期
2 谢宇;金霞;吴胜其;周晓;;Ezrin、CD44在膀胱移行细胞癌的表达与意义[J];现代肿瘤医学;2011年07期
3 李海;张小川;徐松;;大肠腺癌组织中Ezrin蛋白的表达及意义[J];西南军医;2011年04期
4 陈清勇;严杰;胡慧珍;陈方园;宋嘉;吴玉泉;焦德敏;;不同转移潜能人肺癌细胞系Ezrin表达水平及微丝骨架差异性的研究[J];中华肿瘤防治杂志;2011年13期
5 刘津杉;朱明才;董蒲江;;结肠癌中埃滋蛋白和细胞间黏附分子1的表达及意义[J];第三军医大学学报;2011年17期
6 杜丽敏;王如美;时志民;沈利军;李文静;王蕾;;膜细胞骨架连接蛋白Ezrin在乳腺癌转移的表达及意义研究[J];实用心脑肺血管病杂志;2011年10期
7 ;皮肤肿瘤[J];中国医学文摘(皮肤科学);2011年03期
8 ;皮肤肿瘤[J];中国医学文摘(皮肤科学);2011年04期
9 郭建辉;郭雯;赵丽;李双标;;角化棘皮瘤1例[J];中国中西医结合皮肤性病学杂志;2011年04期
10 章纬;张虹亚;;基质金属蛋白酶在皮肤鳞癌侵袭发展中作用的研究进展[J];中国中西医结合皮肤性病学杂志;2011年04期
相关会议论文 前10条
1 郑淑慧;黄静和;向秋玲;周科文;付晓东;王庭槐;;Ezrin蛋白在雌激素促乳腺癌细胞迁移和侵袭中的作用研究[A];中国生理学会第23届全国会员代表大会暨生理学学术大会论文摘要文集[C];2010年
2 范婕;史威;张林西;李海军;金春亭;武欣;郭颖;;乳腺癌中Ezrin表达特点及临床病理意义[A];2009年“临床诊断和治疗新进展研究”暨“全国肿瘤病理诊断交流”研讨会论文集[C];2009年
3 刘林]，
本文编号：2255730
本文链接：https://www.wllwen.com/yixuelunwen/pifb/2255730.html
最近更新
教材专著
