早期梅毒患者TLR2的表达及其与Th细胞亚群的相关性研究

发布时间：2018-10-21 20:17

【摘要】：目的了解未经治疗的早期梅毒患者外周血CD14+单核细胞表面TLR2的表达及其与Th1、Th2细胞的相关性,探究TLR2在梅毒感染中的作用。方法　(1)采用流式细胞术检测45例未经治疗的早期梅毒患者(一期梅毒患者15例,二期梅毒患者15例,早期潜伏梅毒患者15例)和20例正常人外周血CD14+单核细胞表面TLR2的表达;(2)通过用ELISA法检测患者与正常对照血清中IFN-γ、IL-2、IL-4、IL-10的含量,揭示其Th1、Th2细胞的水平。结果　(1)与正常对照组比较,早期梅毒患者(一期、二期、早期潜伏梅毒患者)TLR2高表达(P0.05);其中一期梅毒患者Th1型细胞因子(IFN-γ、IL-2)显著增高(P0.01),二期梅毒患者Th2型细胞因子(IL-10)显著增高(P0.01),早期潜伏梅毒患者各细胞因子无统计学差异(P 0.05);(2)与一期梅毒患者相比,二期、早期潜伏梅毒患者TLR2低表达(P0.05)且Th1型细胞因子(IFN-γ、IL-2)显著下降(P0.01);(3)早期梅毒患者外周血CD14+单核细胞表面TLR2与IFN-γ呈正相关(P0.05),与梅毒血清学滴度(RPR)无相关性(P0.05)。结论　(1)早期梅毒患者体内存在着Th1/ Th2型细胞因子表达的失衡,一期梅毒以Th1型细胞免疫为主,随着梅毒病程的进展,Th1型细胞因子逐渐减少, Th2型细胞因子在增加;(2)TLR2及其介导的天然免疫在梅毒的发生、发展中起重要作用,TLR2与Th1型细胞因子呈正相关,推测TLR2可能参与了早期梅毒患者Th细胞亚群分化,且引起Th1型免疫应答反应。
[Abstract]:Objective to investigate the expression of TLR2 on CD14 monocytes and its correlation with Th1,Th2 cells in untreated early syphilis patients, and to explore the role of TLR2 in syphilis infection. Methods (1) flow cytometry was used to detect 45 cases of untreated early syphilis (15 cases of primary syphilis and 15 cases of secondary syphilis). (2) the expression of TLR2 on the surface of CD14 monocytes in peripheral blood of 15 patients with early latent syphilis and 20 normal controls. (2) the levels of IFN- 纬 and IL-2,IL-4,IL-10 in serum of patients and normal controls were detected by ELISA assay to reveal the level of Th1,Th2 cells. Results (1) compared with the normal control group, the patients with early syphilis (primary, secondary, The expression of TLR2 was high in patients with early latent syphilis (P0.05), IFN- 纬 (IL-2) was significantly higher in primary syphilis patients (P0.01), and Th2 type cytokines (IL-10) was significantly higher in secondary syphilis patients (P0.01), but there was no significant difference among cytokines in patients with early latent syphilis. Compared with patients with primary syphilis, patients with primary syphilis (P0. 05); (2), The low expression of TLR2 (P0.05) and the decrease of IFN- 纬 (IL-2) in patients with early latent syphilis (P0.01); (3) showed a positive correlation between TLR2 and IFN- 纬 on the surface of peripheral CD14 monocytes in early syphilis patients (P0.05), but had no correlation with the serological titer of syphilis (P0.05). Conclusion (1) there is imbalance in the expression of Th1/ Th2 cytokines in early syphilis patients. The primary syphilis is mainly Th1 type cellular immunity. With the progression of syphilis, Th1 type cytokines gradually decreased and Th2 type cytokines increased. (2) TLR2 and its mediated innate immunity played an important role in the development of syphilis, and TLR2 was positively correlated with Th1 type cytokines. It is speculated that TLR2 may be involved in the differentiation of Th cell subsets in early syphilis patients and induce Th1 type immune response.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R759.1

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