磷脂酰肌醇3激酶和蛋白激酶B在小儿血管瘤中的表达及意义

发布时间：2018-11-05 07:28

【摘要】：目的：探讨磷脂酰肌醇3激酶(P13K)、蛋白激酶B(Akt)在小儿血管瘤的发生、发展及消退过程中的作用。方法：收集遵义医学院附属医院2009.01-2012.03住院,未经其他治疗、单纯手术切除并经病理诊断为血管瘤的标本,合Mulliken分类法与增殖细胞核抗原(PCNA)的表达对血管瘤进行分类和分期,抽取增生期与消退期血管瘤各20例,采用免疫组化检测血管瘤组织中磷脂酰肌醇3激酶和蛋白激酶B的表达水平,并进行图像分析。采用定量PCR检测血管瘤组织中磷脂酰肌醇3激酶和蛋白激酶B的(?)nRNA表达情况。结果： 1.20例增殖期血管瘤均表达PI3Kp85、p-Akt,20例退化期血管瘤中PI3Kp85、 p-Akt的表达率分别为14例(70%)、12例(60%),差异均有统计学意义(P0.05)；增殖期血管瘤PI3Kp85(?)勺表达强度为1例(+)、7例(++)、12例(+++),退化期血管瘤PI3Kp85(?)勺表达强度为6例(-)、8例(+)、5例(++)、1例(+++),统计学差异较大(P0.01)；增殖期血管瘤p-Akt的表达强度为2例(+)、11例(++)、7例(+++),退化期血管瘤p-Akt的表达强度为8例(-)、9例(+)、3例(++),统计学差异较大(P0.01)。 2.20例增殖期血管瘤PI3Kp85、p-Akt的积分光密度分别为191.23±40.45、178.73±38.49,退化期血管瘤PI3Kp85、P-Akt的积分光密度分别为46.63±13.08、64.93±21.22,统计学差异均较大(P0.01)。PI3Kp85与p-Akt的表达之间呈正相关关系(r=0.553,P0.05)。 3.20例增殖期血管瘤PI3Kp85mRNA、p-AktmRNA的相对表达值分别为0.0807±0.0136、0.0841±0.0137,退化期血管瘤PI3Kp85、P-Akt的积分光密度分别为0.0203±0.0098、0.0324±0.0178,统计学差异均较大(P0.01)。PI3Kp85mRNA、 P-AktmRNA的表达之间呈正相关关系(r=0.5503P0.01)。结论：小儿增生期和消退期血管瘤组织中PI3Kp85、p-Akt及其mRNA表达有差异；PI3K/Akt信号通路可能在小儿血管瘤的病理演变中发挥作用。
[Abstract]:Objective: to investigate the role of phosphatidylinositol 3 kinase (P13 K) and protein kinase (B (Akt) in the occurrence, development and regression of children hemangioma. Methods: the specimens of hemangioma were collected from the affiliated Hospital of Zunyi Medical College 2009.01-2012.03. Mulliken classification and proliferating cell nuclear antigen (PCNA) expression were used to classify and stage hemangiomas. The expression levels of phosphatidylinositol 3 kinase and protein kinase B in hemangioma tissues were detected by immunohistochemistry and image analysis. The expression of phosphatidylinositol 3 kinase and protein kinase B nRNA was detected by quantitative PCR. Results: 1.The expression rates of PI3Kp85, p-Akt in proliferative hemangioma were 14 cases (70%) and 12 cases (60%), respectively. The difference was statistically significant (P0.05). Proliferative hemangioma PI3Kp85 (?) 1 case of (), 7 cases of (), 12 cases of (), degeneration hemangioma PI3Kp85 (?) The expression intensity of dipper was 6 cases (-), 8 cases of (), was 5 cases of (), 1 case of (), was significantly different (P0.01). The expression intensity of p-Akt in proliferative hemangioma was 2 cases of (), 11 cases of (), 7 cases of (), degenerative hemangioma 8 cases (-) and 9 cases of (), 3 cases of (),. The statistical difference was significant (P0.01). 2. The integral optical density of PI3Kp85,p-Akt was 191.23 卤40.45178.73 卤38.49 in proliferative hemangioma and 46.63 卤13.08 卤64.93 卤21.22 in degenerative hemangioma, respectively. There were significant statistical differences (P0.01). There was a positive correlation between the expression of PI3Kp85 and p-Akt (P 0.05). 3. The relative expression of PI3Kp85mRNA,p-AktmRNA in proliferative hemangioma was 0.0807 卤0.0136 卤0.0137, and the integral optical density of PI3Kp85,P-Akt in degenerative hemangioma was 0.0203 卤0.0098 卤0.0324 卤0.0178, respectively. There were significant statistical differences (P0.01). There was a positive correlation between the expression of PI3Kp85mRNA, P-AktmRNA (r=0.5503P0.01). Conclusion: there are differences in expression of PI3Kp85,p-Akt and mRNA between proliferative and regressive hemangiomas in children, and PI3K/Akt signaling pathway may play a role in the pathological evolution of hemangioma in children.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.5

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