健脾解毒汤对脾虚证银屑病豚鼠模型的实验研究

发布时间：2018-11-13 15:09

【摘要】：目的：初步构建脾虚证银屑病的复合豚鼠模型，并进一步研究健脾解毒汤治疗脾虚证银屑病的相关作用机制及量效关系。方法：将豚鼠按随机数字表分为空白1组，空白2组，模型1组(银屑病组)、模型2组（脾虚型银屑病组）、中药高、中、低剂量组（健脾解毒汤组）、阳性对照组（阿维A胶囊组），除空白1、2组外，其中模型1组采用5%普萘洛尔乳剂涂抹法造成银屑病模型，其余各组均采用病证结合的双重造模法，即用5%普萘洛尔乳剂涂抹法及大黄煎剂灌胃造成脾虚型银屑病豚鼠模型，分别灌胃2周后用ELISA试剂盒检测治疗豚鼠血清TNF-α、IL-20含量的变化并观察其对病理形态学的影响。结果:(1)5%普萘洛尔外涂诱发了豚鼠银屑病样表现，造模后，模型1组与空白1组baker评分有显著差异(P0.01)；灌胃大黄前后，模型2组与空白2组在脾虚证候积分及体重差异显著(P0.01)；(2)健脾解毒汤对脾虚型银屑病样豚鼠模型血清TNF-α和IL-20水平有影响，给药后，，与模型2组相比较，中药高、中剂量组及阳性对照组豚鼠血清TNF-α、IL-20含量明显降低(P0.01)，不同浓度的中药呈现明显的量效关系；(3)健脾解毒汤对脾虚证银屑病样模型组织病理有改变，给药后，中药高、中、低剂量组和阳性对照组对皮损病理组织均有不同程度的改善，与模型2组比较均具有显著统计学差异(P0.01)，并随中药剂量增大其改善作用增强；且中药高剂量组改善略优于阳性对照组(P0.05)；(4)健脾解毒汤对脾虚证候有改善，给药后，与模型2组比较，阳性对照组对体重的影响无统计学差异(P0.05),中药高、中、低剂量组有统计学差异(P0.05)。结论：(1)脾虚证银屑病豚鼠模型具备银屑病病理特征，并与中医脾虚证证候表现相似；(2)健脾解毒汤治疗寻常型银屑病的的机制可能与抑制其炎症反应及免疫调节有关；(3)健脾解毒汤在改善皮损的同时对脾虚征候表现也有所改善。
[Abstract]:Objective: to establish a complex guinea pig model of psoriasis of spleen deficiency syndrome and to study the mechanism and dose-effect relationship of Jianpi jiedu decoction in treating psoriasis of spleen deficiency syndrome. Methods: guinea pigs were randomly divided into blank group 1, blank group 2, model group 1 (psoriasis group), model group 2 (spleen deficiency type psoriasis group), high, middle and low dosage group (Jianpi jiedu decoction group). In the positive control group (Avera capsule group), the psoriasis model was established by 5% propranolol emulsion smear method in model 1 group except for blank group 1, and the other groups were treated with double model combining disease and syndromes. 5% propranolol emulsion smear and rhubarb decoction were used to induce the model of spleen deficiency psoriasis in guinea pigs. The serum TNF- 伪 was detected by ELISA kit for 2 weeks. The changes of IL-20 content and its effect on pathomorphology were observed. Results: (1) Psoriasis was induced by 5% propranolol, and the baker score of model group 1 was significantly different from that of blank group 1 (P0.01). Before and after gavage of rhubarb, there were significant differences between model group 2 and blank group in spleen deficiency syndrome score and body weight (P0.01). (2) Jianpi jiedu decoction had an effect on serum TNF- 伪 and IL-20 levels of psoriatic guinea pigs with spleen deficiency type. Compared with model group 2, serum TNF- 伪 was higher in Chinese herbal medicine group, middle dose group and positive control group. The content of IL-20 was obviously decreased (P0.01), and the dose-effect relationship between different concentrations of Chinese medicine was obvious. (3) Jianpi jiedu decoction changed the pathological changes of psoriasis like model of spleen deficiency syndrome. After administration, the high, middle and low dosage groups and the positive control group all improved the pathological tissue of skin lesions in varying degrees. Compared with the model group 2, there was significant statistical difference (P0.01), and with the increase of the dosage of traditional Chinese medicine, the improvement effect was enhanced. The improvement of high dose group was slightly better than that of positive control group (P0.05). (4) Jianpi jiedu decoction improved the syndrome of spleen deficiency, compared with the model group 2, the positive control group had no significant difference on body weight (P0.05), Chinese medicine high, medium, low dose group had statistical difference (P0.05). Conclusion: (1) Psoriasis guinea-pig model of spleen deficiency syndrome has pathological characteristics of psoriasis and is similar to that of spleen deficiency syndrome in traditional Chinese medicine. (2) the mechanism of Jianpi jiedu decoction in the treatment of psoriasis vulgaris may be related to the inhibition of its inflammatory reaction and immunomodulation; (3) Jianpi jiedu decoction can improve the symptoms of spleen deficiency while improving the skin lesion.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R285.5;R758.63

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