223例男性尖锐湿疣组织HPV基因分型检测及分析

发布时间：2018-11-23 19:22

【摘要】：目的探讨佛山地区男性尖锐湿疣患者生殖器及附近的尖锐湿疣组织中,人乳头瘤病毒的基因型感染情况及其临床意义。方法从223例男性尖锐湿疣患者生殖器及附近的尖锐湿疣组织中提取病毒的基因组DNA,通过聚合酶链式反应扩增后进行导流杂交,利用基因芯片对样本中的HPV DNA进行分型检测。结果 (1)在223例被诊断为尖锐湿疣的受检者中,检出HPV阳性190例,阳性率为85.2%。主要集中在21~40岁年龄段。(2)在190例HPV阳性病例中,单一感染137例,占72.1%;多重感染53例,占27.9%。在HPV单一亚型感染病例中,6、11型是主要感染亚型。在HPV多重感染病例中,共出现5种多重感染类型,其中二重感染为主要类型。在多重感染病例中,主要为高危型和低危型同时存在的混合型感染。结论佛山地区男性尖锐湿疣患者HPV感染主要以6、11型为主,好发于21~40岁的青壮年阶段,以单一感染为主,多重感染则以混合型感染为主。
[Abstract]:Objective to investigate the genotypic infection of human papillomavirus (HPV) in the genitals of male patients with condyloma acuminatum in Foshan area and its clinical significance. Methods Genomic DNA, extracted from genitals and adjacent condyloma acuminatum tissues of 223 male condyloma acuminatum patients were amplified by polymerase chain reaction (PCR) and then hybridized by diversion hybridization. The HPV DNA in the samples were detected by gene chip. Results (1) out of 223 cases diagnosed as condyloma acuminatum, 190 cases were positive for HPV, the positive rate was 85.2%. (2) of the 190 HPV positive cases, 137 cases were single infection (72.1%), 53 cases were multiple infection (27.9%). In the single subtype of HPV infection, type 6M11 is the main subtype of infection. In HPV multiple infection cases, there are 5 multiple infection types, of which double infection is the main type. In multiple infection cases, high-risk type and low-risk type of mixed infection exist simultaneously. Conclusion HPV infection in male patients with condyloma acuminatum in Foshan area is mainly of type 6: 11, which mainly occurs at the age of 21 to 40 years, with a single infection and multiple infections with a mixed infection.
【作者单位】：广东省佛山市第二人民医院检验科;
【分类号】：R752.53

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