Neuropilin-1在婴幼儿皮肤血管瘤中的表达及意义

发布时间：2018-12-13 01:47

【摘要】：目的探讨Neuropilin-1在婴幼儿皮肤血管瘤增生期、消退期组织中的表达及意义。材料与方法收集华中科技大学附属协和医院2007年1月—2010年3月间切取的47例患者的血管瘤组织作为实验组,术前均未行其他治疗并经病理检查和追溯病史证实为婴幼儿血管瘤。对照组为我科在手术时所留取的含血管的正常皮肤组织,共10例。根据HE染色结果将血管瘤分为增生期组与消退期组,采用免疫组化SABC技术,检测26例增生期婴幼儿血管瘤、21例消退期及10例正常皮肤组织中Neuropilin-1蛋白的表达,应用Image-pro plus6.0软件测量各标本Neuropilin-1蛋白表达的积分光密度(IOD)、平均光密度,结果用SPSS12.0统计软件进行数据分析。结果47例婴幼儿血管瘤标本中增生期组、消退期组Neuropilin-1均呈阳性表达,正常皮肤血管内皮细胞未见明显表达。增生期血管瘤中Neuropilin-1的表达高于消退期和正常皮肤,其差异有显著性(P0.05);消退期表达水平高于正常皮肤组织,其差异有显著性(P0.05)。结论Neuropilin-1在婴幼儿血管瘤中高表达,增生期与消退期表达水平存在显著差异,可能是血管瘤内皮细胞增殖的重要促进因子。
[Abstract]:Objective to investigate the expression and significance of Neuropilin-1 in the proliferative and involutive phases of infantile skin hemangioma. Materials and methods the hemangioma tissues of 47 patients were collected from Union Hospital of Huazhong University of Science and Technology from January 2007 to March 2010 as experimental group. No other treatment was performed before operation and proved to be infantile hemangioma by pathological examination and retrospective history. The control group consisted of 10 cases of normal skin tissue containing blood vessels. According to the results of HE staining, hemangiomas were divided into proliferative phase group and regression phase group. The expression of Neuropilin-1 protein in 26 cases of proliferative phase hemangioma, 21 cases of regression phase and 10 cases of normal skin tissue were detected by immunohistochemical SABC technique. The integral optical density (IOD),) of Neuropilin-1 protein was measured by Image-pro plus6.0 software. The results were analyzed by SPSS12.0 software. Results in 47 cases of infantile hemangioma, the positive expression of Neuropilin-1 was found in proliferative phase group and regression phase group, but no obvious expression was found in normal skin vascular endothelial cells. The expression of Neuropilin-1 in proliferative hemangioma was higher than that in involutive phase and normal skin (P0.05), and the expression level in involutive phase was higher than that in normal skin tissue (P0.05). Conclusion the high expression of Neuropilin-1 in infantile hemangioma and the significant difference between proliferative phase and regression phase may play an important role in the proliferation of endothelial cells in hemangioma.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.5

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