云南汉族多形性日光疹与HLA-DQA1、DQB1等位基因相关性研究
发布时间：2018-12-14 18:54
【摘要】：目的探讨多形性日光疹(Polymorphic light eruption,PLE)与HLA-DQA1、DQB1等位基因的相关性,拟明确PLE可能的HLA易感等位基因和(或)保护性基因。 方法采用经典的酚-氯仿法从110例PLE患者和170例健康对照的外周静脉血白细胞中提取基因组DNA,应用聚合酶链反应-直接测序(polymerase chain reaction sequence-based typing,PCR-SBT)的方法对PLE患者和健康对照组进行HLA-DQA1、DQB1等位基因的检测。 结果与对照组相比,病例组中HLA-DQA1*0102,DQB1*0302和DQB1*0601(OR=3.000,Pc=0.0045；OR=7.485,Pc10-3；OR=14.153,Pc10-4)等位基因频率显著升高,而DQB1*0201频率显著降低(OR=0.354,Pc=0.0280)。 结论HLA-DQA1*0102,DQB1*0302和DQB1*0601可能是云南汉族PLE的易感基因或与实际的易感基因连锁,而HLA-DQB1*0201可能为云南汉族PLE的保护性基因。
[Abstract]:Objective to investigate the relationship between (Polymorphic light eruption,PLE and HLA-DQA1,DQB1 alleles and to identify the possible HLA susceptibility alleles and / or protective genes in PLE. Methods Genomic DNA, was extracted from peripheral venous leukocytes of 110 patients with PLE and 170 healthy controls by classical phenol-chloroform method. Polymerase chain reaction-direct sequencing (polymerase chain reaction sequence-based typing, was used. PCR-SBT was used to detect HLA-DQA1,DQB1 alleles in PLE patients and healthy controls. Results compared with the control group, HLA-DQA1*0102,DQB1*0302 and DQB1*0601 (OR=3.000,Pc=0.0045;OR=7.485,Pc10-3;) were found in the case group. The allele frequency of OR=14.153,Pc10-4 increased significantly, while the frequency of DQB1*0201 decreased significantly (OR=0.354,Pc=0.0280). Conclusion HLA-DQA1*0102,DQB1*0302 and DQB1*0601 may be susceptible genes of PLE or linked to actual susceptibility genes in Yunnan Han nationality, while HLA-DQB1*0201 may be the protective gene of PLE in Yunnan Han nationality.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R758.14
本文编号：2379145
[Abstract]:Objective to investigate the relationship between (Polymorphic light eruption,PLE and HLA-DQA1,DQB1 alleles and to identify the possible HLA susceptibility alleles and / or protective genes in PLE. Methods Genomic DNA, was extracted from peripheral venous leukocytes of 110 patients with PLE and 170 healthy controls by classical phenol-chloroform method. Polymerase chain reaction-direct sequencing (polymerase chain reaction sequence-based typing, was used. PCR-SBT was used to detect HLA-DQA1,DQB1 alleles in PLE patients and healthy controls. Results compared with the control group, HLA-DQA1*0102,DQB1*0302 and DQB1*0601 (OR=3.000,Pc=0.0045;OR=7.485,Pc10-3;) were found in the case group. The allele frequency of OR=14.153,Pc10-4 increased significantly, while the frequency of DQB1*0201 decreased significantly (OR=0.354,Pc=0.0280). Conclusion HLA-DQA1*0102,DQB1*0302 and DQB1*0601 may be susceptible genes of PLE or linked to actual susceptibility genes in Yunnan Han nationality, while HLA-DQB1*0201 may be the protective gene of PLE in Yunnan Han nationality.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R758.14
【参考文献】
相关期刊论文 前3条
1 范丽安,周光炎;人类基因组MHC测序及其免疫学意义[J];上海免疫学杂志;1999年06期
2 邓丹琪,韩云涛,陈浩,李海英;云南省不同地区多形性日光疹、慢性光线性皮炎患病率调查[J];中华皮肤科杂志;2005年07期
3 ;Polymorphism of the second exon of human leukocyte antigen-DQA1, -DQB1 gene and genetic susceptibility to idiopathic dilated cardiomyopathy in people of the Han nationality in northern China[J];Chinese Medical Journal;2005年03期
，本文编号：2379145
本文链接：https://www.wllwen.com/yixuelunwen/pifb/2379145.html
最近更新
教材专著
