HIPK2和CYCLIN D1在鲍温病和皮肤鳞状细胞癌中的表达

发布时间：2019-01-20 19:24

【摘要】： 目的：检测同源结构域相互作用蛋白激酶2(HIPK2)和细胞周期蛋白D1(cyclin D1)在鲍温病和鳞状细胞癌中的表达,以探讨它们在肿瘤发生发展中的作用与关系。方法：鲍温病(BD)和鳞状细胞癌(SCC)的皮损标本来源于2007年至2010年复旦大学附属华山医院皮肤科门诊病人,共计54例,并经组织病理确诊。其中24例为皮肤鳞状细胞癌,30例为鲍温病,8例为正常对照。取患者皮损组织用于常规病理分析并采用免疫组化学方法检测鲍温病组织、鳞状细胞癌组织、正常皮肤组织中HIPK2和cyclin D1的表达水平。结果：在鲍温病组中HIPK2蛋白阳性率为90%(27／30),在鳞状细胞癌组中HIPK2的阳性率为91.7%(22／24),在对照组HIPK2蛋白阳性率为100%(8／8)。HIPK2的表达在鳞状细胞癌组及鲍温病组中与正常对照组相比,差异无显著性(p0.05)；鲍温病组与鳞状细胞癌组间差异无显著性意义(p0.05)。但从病理角度来看,HIPK2在表皮基底细胞增殖层细胞核内表达,而在表皮非增殖层它的表达逐渐消失。HIPK2在鲍温病中表达主要位于非典型增生的区域的细胞核内而在鳞状细胞癌中表达在癌巢边细胞和癌巢中央细胞。Cyclin D1的阳性率在鲍温病组中为43.3%(13／30),在鳞状细胞癌组中cyclin D1的阳性率为70.8%(17／24),在对照组cyclin D1阳性率为0%(0／8)。在鳞状细胞癌组及鲍温病组中cyclin D1的表达比正常对照组明显增高,差异有统计学意义,且鲍温病组与鳞状细胞癌组间差异有统计学意义(p0.05)。HIPK2和cyclin D1的表达与鳞状细胞癌的病理分化程度差异无统计学意义(p0.05)。在鲍温病组及状细胞癌组中cyclin D1与HIPK2两者分布无相关性。结论：HIPK2的表达在皮肤肿瘤中可能起着细胞增殖作用而不是生长抑制功能。Cyclin D1的表达在皮肤细胞的癌变过程中为一早期分子,对皮肤肿瘤发生、发展有重要作用,而且cyclin D1在鳞状细胞癌中的表达不受分化程度的影响。
[Abstract]:Aim: to investigate the expression of homologous domain interacting protein kinase 2 (HIPK2) and cyclin D1 (cyclin D1) in Baldwin's disease and squamous cell carcinoma (SCC). Methods: the lesions of (BD) and (SCC) from 2007 to 2010 were collected from 54 outpatients in the Department of Dermatology affiliated to Fudan University and confirmed by histopathology. There were 24 cases of cutaneous squamous cell carcinoma, 30 cases of Bowen's disease and 8 cases of normal control. The expressions of HIPK2 and cyclin D1 were detected by immunohistochemical method in the tissues of Balwin's disease, squamous cell carcinoma and normal skin. Results: the positive rate of HIPK2 protein was 90% (27 / 30) in Bowen's disease group and 91.7% (22 / 24) in squamous cell carcinoma group. The positive rate of HIPK2 protein in the control group was 100% (8 / 8). There was no significant difference in the expression of HIPK2 between the squamous cell carcinoma group and the Bowen disease group compared with the normal control group (p0. 05). There was no significant difference between Bowen disease group and squamous cell carcinoma group (p 0.05). However, from the pathological point of view, HIPK2 was expressed in the nucleus of epidermal basal cell proliferating layer. In the epidermal non-proliferative layer, the expression of HIPK2 was mainly located in the nucleus of atypical proliferative region and in squamous cell carcinoma. The positive expression of Cyclin D1 was found in the nesting cell and the central cell of the cancer nest. The sex rate in the Bowen group was 43.3% (13 / 30). The positive rate of cyclin D1 was 70.8% (17 / 24) in squamous cell carcinoma group and 0% (0 / 8) in control group. The expression of cyclin D1 in squamous cell carcinoma group and Bowen disease group was significantly higher than that in normal control group, and the difference was statistically significant. The expression of HIPK2 and cyclin D1 was not significantly different from that of squamous cell carcinoma (p0.05). There was no correlation between cyclin D1 and HIPK2 in patients with Bowen's disease and in patients with HCC. Conclusion: the expression of HIPK2 may play a role in cell proliferation rather than growth inhibition in skin tumors. The expression of Cyclin D1 may be an early molecule in the carcinogenesis of skin cells, and may play an important role in the occurrence and development of skin tumors. Moreover, the expression of cyclin D1 in squamous cell carcinoma is not affected by the degree of differentiation.
【学位授予单位】：复旦大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.5

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