诱发儿童特应性皮炎的南美白对虾过敏原的研究

发布时间：2019-02-22 13:56

【摘要】：目的：研究南美白对虾蛋白质中主要致敏片段与儿童特应性皮炎(AD)发病的相关性。方法：对158例AD患儿进行虾过敏原的点刺试验,筛选阳性患儿46例进行南美白对虾口服激发试验,确定36例患儿对其过敏并作为实验组,留取实验组患儿血清作为抗体与南美白对虾全蛋白进行免疫印迹检测,找到诱发儿童AD的南美白对虾蛋白质中主要致敏片段；对照组同时进行上述实验。结果：实验组36例患儿血清免疫印迹结果显示：32例血清出现明显的阳性条带,4例为阴性；阳性条带蛋白质片段的分子量分别为20kDa、36kDa和40.1kDa；其中,三条条带均阳性6例,两条条带阳性7例,单条带阳性19例；分子量为36kDa蛋白质片段阳性为29例,分子量为20kDa和40.1kDa蛋白质片段阳性各为11例。对照组36例血清免疫印迹检测结果均为阴性。将分子量为36kDa蛋白质片段分别与分子量为20kDa和40.1kDa蛋白质片段进行阳性率比较,阳性率差别均有统计学意义(x2均为14.09,p均小于0.001)；对分子量为20kDa与40.1kDa的蛋白质片段进行阳性率比较,差别无统计学意义(x2=0,p=1.00)。结论：分子量为36kDa蛋白质片段(原肌球蛋白)为南美白对虾全蛋白中主要的致敏片段；推测南美白对虾全蛋白中分子量为36kDa蛋白质片段(原肌球蛋白)可能为诱发儿童特应性皮炎的主要致敏片段。
[Abstract]:Objective: to study the correlation between the main sensitized fragments in protein of Penaeus vannamei and the pathogenesis of (AD) in children with atopic dermatitis. Methods: a prick test of shrimp allergens was carried out in 158 cases of AD, 46 cases of positive children were tested by oral excitation test, 36 cases of children were determined to be allergic to them and used as experimental group. The serum of the experimental group was used as the antibody and the whole protein of Penaeus vannamei was detected by Western blotting, and the main sensitized fragment of the protein of Penaeus vannamei induced by AD in children was found. In the control group, the experiment was carried out simultaneously. Results: the results of serum immunoblotting showed that 32 cases had obvious positive bands, 4 cases were negative, the molecular weight of the protein fragments were 20kDa 36kDa and 40.1kDa, respectively. Among them, 3 bands were positive in 6 cases, 2 bands were positive in 7 cases, single band was positive in 19 cases, molecular weight of 36kDa protein fragment was positive in 29 cases, and molecular weight of 20kDa and 40.1kDa protein fragment was positive in 11 cases respectively. The results of serum immunoblotting were negative in 36 cases of control group. The positive rates of 36kDa protein fragments with molecular weight of 20kDa and 40.1kDa protein fragments were significantly higher than those of 20kDa and 40.1kDa protein fragments (x2 = 14.09, p < 0.001). The positive rates of protein fragments with molecular weight of 20kDa and 40.1kDa were not significantly different (x2 + 0% p1. 00). Conclusion: the molecular weight of 36kDa protein fragment (promyosin) is the main sensitized fragment in the whole protein of Penaeus vannamei. The molecular weight of the whole protein of Penaeus vannamei (Penaeus vannamei) is 36kDa protein fragment (promyosin) which may be the main sensitizing fragment to induce atopic dermatitis in children.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R758.29

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