宁夏地区回族和汉族白癜风患者与HLA-DRB1的相关性探讨
发布时间：2019-03-16 15:53
【摘要】：目的探究宁夏地区回汉族白癜风患者与HLA-DRB1等位基因的相关性,以及回汉族人群在HLA-DRB1等位基因频率分布的差异性,为揭示宁夏回汉族人群白癜风发病机理及遗传特点提供可靠实验室依据。 方法采用序列特异性引物-聚合酶链反应(PCR-SSP)方法,对153例白癜风患者及198例对照组进行HLA-DRB1等位基因检测,使用SPSSl3.0统计软件,显著性用χ2检验,P0.05认为有显著性差异。 结果①所检测的HLA-DRB1的12个等位基因在宁夏地区回族正常人与汉族正常人的分布中无统计学差异。②宁夏汉族白癜风患者较汉族正常对照组HLA-DRB1*09基因频率显著增高(9.2%vs2.5%),RR=4.222,χ2=9.787,Pc0.05。③宁夏汉族泛发型白癜风患者较汉族正常对照组HLA-DRB1*09基因频率显著增高(9.4%vs2.5%),RR=4.308,χ2=9.390, Pc0.05。④宁夏回族白癜风患者较回族正常对照组HLA-DRB1*16基因频率显著增高(10%vs1.3%),RR=9.750,χ2=9.255, Pc0.05。⑤宁夏回族泛发型白癜风患者较汉族正常对照组HLA-DRB1*16基因频率显著增高(11.4%vs1.3%),RR=11.556,χ2=10.274, Pc0.05。⑥宁夏回汉族局限型白癜风患者较回汉族正常对照组相比无统计学意义。 结论HLA-DRB1*09可能是宁夏汉族白癜风患者尤其是泛发型患者的易感基因,或与易感基因相连锁。HLA-DRB1*16可能是宁夏回族白癜风患者尤其是回族泛发型患者的易感基因,或与易感基因相连锁。
[Abstract]:Objective to investigate the association between HLA-DRB1 allele and vitiligo in Hui Han population in Ningxia and the difference of frequency distribution of HLA-DRB1 allele in Hui Han population. To provide a reliable laboratory basis for revealing the pathogenesis and genetic characteristics of vitiligo in Ningxia Hui Han population. Methods sequence specific primer polymerase chain reaction (PCR-SSP) was used to detect HLA-DRB1 alleles in 153 patients with vitiligo and 198 controls. The statistical software SPSSl3.0 was used and the significance was tested by 蠂 2 test. There was significant difference between the two groups (P0.05). Results 1 there was no significant difference in the distribution of 12 alleles of HLA-DRB1 between Hui and Han people in Ningxia area. 2 the frequency of HLA-DRB1*09 gene in Ningxia Han nationality patients with vitiligo was higher than that in Han nationality control group. The rate was significantly higher (9.2% vs 2.5%), The frequency of HLA-DRB1*09 gene in Ningxia Han nationality patients with generalized vitiligo was significantly higher than that in Han control group (9.4% vs 2.5%) and RR=4.308, 蠂 2 / 9.390,9.787, RR=4.222, 蠂 2 / 9.787, Pc0.05.3, P < 0.05, P < 0.01, respectively. The frequency of HLA-DRB1*16 gene in Ningxia Hui patients with vitiligo in Pc0.05.4 was significantly higher than that in the control group (10% vs 1.3%), and RR=9.750, 蠂 2 was 9.255, The frequency of HLA-DRB1*16 gene in Pc0.05.5 Hui nationality patients with generalized vitiligo in Ningxia was significantly higher than that in Han control group (11.4% vs 1.3%), and RR=11.556, 蠂 2 was 10.274, There was no significant difference in Pc0.05.6 between Ningxia Hui Han nationality patients with localized vitiligo and Hui Han normal controls. Conclusion Hla-DRB1*16 may be a susceptible gene in Ningxia Han nationality patients with vitiligo, especially in patients with generalized vitiligo. Hla-DRB1*16 may be a susceptible gene in Hui patients with vitiligo, especially those with generalized type of vitiligo in Ningxia. HLA-DRB1*09-DRB1*16 may be a susceptible gene in Hui patients with vitiligo, especially in patients with generalized vitiligo. Or linked to susceptible genes.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R758.41
[Abstract]:Objective to investigate the association between HLA-DRB1 allele and vitiligo in Hui Han population in Ningxia and the difference of frequency distribution of HLA-DRB1 allele in Hui Han population. To provide a reliable laboratory basis for revealing the pathogenesis and genetic characteristics of vitiligo in Ningxia Hui Han population. Methods sequence specific primer polymerase chain reaction (PCR-SSP) was used to detect HLA-DRB1 alleles in 153 patients with vitiligo and 198 controls. The statistical software SPSSl3.0 was used and the significance was tested by 蠂 2 test. There was significant difference between the two groups (P0.05). Results 1 there was no significant difference in the distribution of 12 alleles of HLA-DRB1 between Hui and Han people in Ningxia area. 2 the frequency of HLA-DRB1*09 gene in Ningxia Han nationality patients with vitiligo was higher than that in Han nationality control group. The rate was significantly higher (9.2% vs 2.5%), The frequency of HLA-DRB1*09 gene in Ningxia Han nationality patients with generalized vitiligo was significantly higher than that in Han control group (9.4% vs 2.5%) and RR=4.308, 蠂 2 / 9.390,9.787, RR=4.222, 蠂 2 / 9.787, Pc0.05.3, P < 0.05, P < 0.01, respectively. The frequency of HLA-DRB1*16 gene in Ningxia Hui patients with vitiligo in Pc0.05.4 was significantly higher than that in the control group (10% vs 1.3%), and RR=9.750, 蠂 2 was 9.255, The frequency of HLA-DRB1*16 gene in Pc0.05.5 Hui nationality patients with generalized vitiligo in Ningxia was significantly higher than that in Han control group (11.4% vs 1.3%), and RR=11.556, 蠂 2 was 10.274, There was no significant difference in Pc0.05.6 between Ningxia Hui Han nationality patients with localized vitiligo and Hui Han normal controls. Conclusion Hla-DRB1*16 may be a susceptible gene in Ningxia Han nationality patients with vitiligo, especially in patients with generalized vitiligo. Hla-DRB1*16 may be a susceptible gene in Hui patients with vitiligo, especially those with generalized type of vitiligo in Ningxia. HLA-DRB1*09-DRB1*16 may be a susceptible gene in Hui patients with vitiligo, especially in patients with generalized vitiligo. Or linked to susceptible genes.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R758.41
【参考文献】
相关期刊论文 前10条
1 卢宁;高天文;姚庆君;赵明;李凯;杨励;高超;;白癜风人群雌激素受体基因多态性与临床资料相关性分析[J];第四军医大学学报;2007年04期
2 周凯;张成锋;王建新;李冰;朱健;;建鲤MHC classⅠ基因全长cDNA的克隆与序列分析[J];淡水渔业;2011年01期
3 魏华;HLA分型进展[J];国外医学(免疫学分册);2003年01期
4 常建民,冯和平,杨敏,杜娟,朱铁君;寻常型白癜风患者血浆内皮素-1的测定[J];临床皮肤科杂志;2000年05期
5 王s，
本文编号：2441680
本文链接：https://www.wllwen.com/yixuelunwen/pifb/2441680.html
最近更新
教材专著
